Table 1.

ADP-regulated kinases and Tyr-phosphatases

Early kinases (10 s)
FamilyUniprotGene (alternative)SiteRegulation
AGCQ05655PRKCD (PKDCδ)Ser130↑10ADP
Thr218↑10ADP
Ser304↑10ADP
AGCQ04759PRKCQ (PKCθ)Thr219↑10ADP
Ser685↑10ADP
AGCQ13464ROCK1Ser1341↑10ADP
AGCO75116ROCK2Ser1374↑10ADP
AGCP23443RPS6KB1 (p70S6K)Ser427↑10ADP
CAMKO60229KALRN (TRAD)Ser1896↑10ADP
Ser2261↓10ADP
Ser1756+1757↑10ADP
CAMKP49137MAPKAPK2Thr334↑10ADP
CAMKQ9BUB5MKNK1 (MNK1)Thr385↑10ADP
CAMKQ7KZI7MARK2Thr596↑10ADP
CAMKQ15746MYLK (smMLCK)Ser1760↑10ADP
Ser1776/Ser1779↑10ADP
CAMKQ13555CAMK2GThr287↑10ADP
Thr306↑10ADP
Ser311↑10ADP
CK1P0C1S8WEE2 (WEE1B)Ser139↑10ADP
CMGCO94921CDK14 (PFTAIRE1)Ser24↑10ADP
STEO94804STK10 (LOK)Ser454↑10ADP
STEQ13043STK4 (MST1)Ser414↑10ADP
Ser438↑10ADP
STEQ99683MAP3K5 (ASK)Ser1033↑10ADP
Ser1240↑10ADP
Ser1029+Ser1033↑10ADP
STEQ13177PAK2Ser19/Ser20↑10ADP
TKP52333JAK3Ser15/17/20/Thr21↑10ADP
TKQ06187BTKThr184↑10ADP
TKQ07912TNK2 (ACK)Thr517/Tyr518↑10ADP
TKLQ8IVT5KSR1Thr268+Thr272↓10ADP
Q8TD19NEK9Thr333↑10ADP
Q9H4A3WNK1Ser1261↓10ADP
Medium (30 s) and late kinases (60 s)
FamilyUniprotGene (alternative)SiteRegulation
AGCQ05655PRKCD (PKCδ)Thr43↑30ADP
CAMKQ9Y2K2SIK3 (QSK)Ser568↓30ADP
CAMKQ9UEW8STK39 (PASK)Ser385↓30ADP
STEQ13043STK4 (MST1)Thr340↑60ADP
STEQ7L7X3TAOK1 (TAO1)Thr440↑30ADP
STEQ9UKE5TNIK (ZC2)Thr903↑60ADP
STEQ13233MAP3K1 (MEKK1)Thr20↓60ADP
STEQ8N4C8MINK1 (MINK/ZC3)Ser701↓60ADP
STEQ9Y4K4MAP4K5 (KHS1)Thr168↑60ADP
STEO95747OXSR1 (OSR1)Ser359↓60ADP
STEQ13177PAK2Ser197↑30ADP
TKP43405SYKSer297↑30ADP
TKLP15056BRAFSer151↓60ADP
Tyrosine phosphatases
UniprotGeneSiteRegulation
Q05209PTPN12Thr454↓10ADP
Q99952PTPN18Ser341↑10ADP
Q8WYL5SSH1Ser897↑10ADP
Q8TE77SSH3Ser37↑10ADP
Q8TF42UBASH3BThr106↑10ADP
Regulatory subunits of phosphatases
UniprotGeneSiteRegulation
O14974PPP1R12ASer478/Ser479↑10ADP
Ser903↑60ADP
O95685PPP1R3DSer25↑10ADP
Q96SB3PPP1R9BSer99/Ser100↑10ADP
  • Kinase phosphorylation sites were grouped into early (10 seconds), medium (30 seconds), and late (60 seconds) responders. ↑10ADP corresponds to upregulation after 10 seconds of ADP stimulation. Involvement of Tyr-phosphatases and regulatory subunits are summarized. If no specific phosphorylation Ser/Thr/Tyr residue could be assigned (eg, JAK3), all possible phospho-residues are listed.