Table 1

Increased colony forming capacity of BM or spleen cells from Tet2−/− mice

GenotypeCellsCFU-GMBFU-ECFU-GEMMTotal
WT Control (n = 6)BM32 ± 22 ± 11 ± 135 ± 5
SP10 ± 21 ± 1011 ± 3
Tet2−/− (2-4 months; n = 8)BM52 ± 12*3 ± 23 ± 158 ± 13*
SP64 ± 8**4 ± 2*0 ± 168 ± 10**
Tet2−/− (erythroid infiltration; n = 6)BM1 ± 1**12 ± 8**013 ± 7
SP6 ± 222 ± 10**028 ± 14
Tet2−/− or Tet2−/− myeloid infiltration (n = 5)BM48 ± 10**02 ± 150 ± 11**
SP26 ± 7**0 ± 1027 ± 8**
  • In vitro hematopoietic colony-forming assays were performed with BM (1 × 104) and spleen (SP; 1 × 105) cells from indicated genotypes of mice in the presence of mSCF, mIL-3, IL-6, and EPO. Colonies were enumerated on day 8 of culture. Data are presented as mean ± SEM.

  • * P < .05,

  • ** P < .01.