Table 3.

Cytokine production after stimulation of human T cells from spleen and thymus

ExperimentIL-2 (pg/mL)IL-4 (pg/mL)IFN-γ (pg/mL)
144.53.0316.5
213.321.9289.0
3157.513.5424.0
  • Human cells were cultured from murine spleen by stimulation with PHA. After washing, these cells were stimulated with anti-CD3 + TPA or ConA. Supernatants were harvested 24 hours later, and cytokine content was measured. Data shown are from 3 mice injected from the same UCB donor, and numbers shown are averages from 2 stimulation methods.