Table 1.

Hematologic parameters of Stat5a−/−5b−/− mice

AgeGenotype
(no. of animals)
Mean ± SEMP
Hematocrit (%)Neonates5a−/−5b−/−(65)28.9 ± 0.9*, * P< .0001
P < .0001
5a+/−5b+/−(88)41.3 ± 0.5* * P = .0014
Wild-type (12)47.6 ± 1.6
3 wk5a−/−5b−/−(12)32.6 ± 1.0*, * P< .0001
P = .03
5a+/−5b+/−(23)36.5 ± 1.2* * P = .0006
Wild-type (5)45.7 ± 1.2
5-12 wk5a−/−5b−/−(26)27.9 ± 2.0* * P < .0001
5a+/−5b+/−(3)29.5 ± 6.2* * P = .008
Wild-type (15)45.2 ± 0.9
3-6 mo5a−/−5b−/−(21)41.1 ± 1.9* * P = .0014
5a+/−5b+/−(5)44.0 ± 2.6
Wild-type (15)49.2 ± 1.0
MCV (μ3)Neonates5a−/−5b−/−(14)89.7 ± 1.6*, * P< .0001
P = .0003
5a+/−5b+/−(25)97.3 ± 1.25
Wild-type (9)102.8 ± 1.5
3 wk5a−/−5b−/−(10)56.1 ± 0.73*, * P= .004
P = .002
5a+/−5b+/−(18)59.1 ± 0.45
Wild-type (5)60.0 ± 1.47
5 wk5a−/−5b−/−(10)51.8 ± 2.75
5a+/−5b+/−(18)54.6 ± 1.1
Wild-type (5)54.6 ± 1.3
Total bilirubin (mg/dL)>6 wk5a−/−5b−/−(9)0.46 ± 0.19
Wild-type (6)0.12 ± 0.02
LDH (IU/L)5a−/−5b−/−(9)715 ± 372
Wild-type (6)282 ± 120
Serum iron (μg/dL)5a−/−5b−/−(6)161.3 ± 15.1
Wild-type (6)152.7 ± 17.9
TIBC (μg/dL)5a−/−5b−/−(6)617 ± 40.0* * P < .0001
Wild-type (6)427 ± 18.8
  • All measurements were carried out on Stat5a−/−5b−/− mice of the ages indicated, and on Stat5a−/−5b−/−, Stat5a+/−5b+/− or wild-type controls from the same mouse colony. Hematocrit data corresponds to Figure 1.P values are indicated only when P < .05. The increase in total iron-binding capacity (TIBC) in Stat5a−/−5b−/− mice is consistent with ineffective erythropoiesis. Red cell indices in Stat5a−/−5b−/− mice other than MCV were not significantly different than control values. P was calculated using Fisher protected least significant difference (PLSD) post hoc analysis.

  • * Significantly different from wild-type value.

  • Significantly different from Stat5a+/−5b+/− value.