Table 1.

Depletion of NK cells using anti-asialo GM1 antibody in BALB/c.B6Cmv1 rmice

Tissue*nNo. leukocytes
(×10−6)
% NK1.1+
NK cells
% TCRβ+
cells
% NK1.1+
TCRβ+cells
Spleen5105 ± 51-153 3.0 ± 0.425 ± 20.4 ± 0.1
Spleen + Ab390 ± 7< 0.226 ± 30.5 ± 0.2
Liver52.4 ± 0.419.7 ± 1.443 ± 47.7 ± 1.3
Liver + Ab32.8 ± 0.10.3 ± 0.144 ± 47.5 ± 3.4
  • Cell suspensions were prepared as described and labeled with mAb specific for αβTCR and NK1.1.

  • * Mice were untreated or treated with 100 μg anti-asialo GM1 one day before tissue harvest.

  • n, number of mice tested.

  • Total number of leukocytes isolated from spleen or liver.21

  • F1-153 Results represent mean ± SE.