Table 3.

Lack of effect of short-term exposure to TGF-β1 on the repopulating activity of cultured CD34+ cord blood cells

Experiment no.Suspension culture
+ 5 GF
Suspension culture
+ 5 GF + TGF-β1
BM stromaBM stroma + 5 GFBM stroma + TGF-β1
Cells per
mouse3-150
(×106)
L+ + M+Cells per
mouse3-150
(×106)
L+ + M+Cells per
mouse3-150
(×106)
L+ + M+Cells per
mouse3-150
(×106)
L+ + M+Cells per
mouse3-150
(×106)
L+ + M+
13.0 7/82.1 7/81.5 8/82.1 7/80.9 6/6
23.3 5/101.6 7/100.8 3/91.8 3/100.9 4/7
31.5 8/81.2 5/80.8 5/71.2 6/60.8 3/5
Total20/26 (77%)19/26 (73%)16/24 (67%)16/26 (62%)13/18 (72%)
  • TGF-β1 indicates transforming growth factor β1; BM, bone marrow.

  • F3-150 More than 90% of all the cells from each culture were injected into a minimum of 8 irradiated NOD/SCID mice in each experiment. 5GF = FL + SF + IL-3 + IL-6 + G-CSF, as described in “Materials and methods.”