Table 1.

Differential repopulating activity of G0/G1 and S/G2/M cells present in 5-day cultures of CD34+ cord blood cells

Experiment no.G0/G1S/G2/M
Cells per mouse* (×106)Fraction of positive miceCells per mouse*(×106)Fraction of positive mice
L+ + M+ (% human cells)L+ ± M+L+ + M+L+ ± M+
11.52/6  (0.6, 0.3)6/60.730/64/6
21.06/6  (1.3, 10.9, 0.7, 1.5, 0.6, 1.5)6/60.650/60/6
30.783/5  (1.1, 0.2, 0.6)5/50.460/50/5
  • * More than 90% of all cells in each fraction from the entire culture were injected into 6 irradiated NOD/SCID mice in each experiment.

  • % of human CD45/CD71+ cells in the bone marrow of those mice found to be positive 6 weeks after transplant (L+= at least 5 of 20 000 cells were human CD34CD19/20+, M+ = at least 5 of 20 000 cells were human CD45/71+CD15/66b+cells).