Table 4.

MLL Gene Rearrangements Detected by Southern Blot Analysis

Patients Molecular Analysis of MLL Gene
HindIII PS/98/4.2 EcoRI PS/98/4.2 BamHI PS/98/4.2
;A.  t(4;11)+, std PCR, nested PCR+
  INF-6 R/R/R R/G/GR/R/R
  PEDS-87 G/R/G R/G/G G/G/G
;B.  t(4;11), std PCR, nested PCR+
  PEDS-27 R/G/RG/G/G R/G/G
  PEDS-44 R/G/R G/G/GR/G/R
  PEDS-59 NE G/G/G NE
  PEDS-5 NE/R/NE R/G/NE R/R/R
  PEDS-106 G/G/G G/G/G NE
;C.  t(4;11)+, std PCR+, nested PCR+
  INF-12 R/R/R G/G/GR/G/R
  PEDS-91 G/R/G G/R/G G/G/NE
D. Normal cells
  FL-12 (21 wk) G/G/GG/G/G G/G/G
  FL-14 (19 wk) G/G/G G/G/GG/G/G
  Normal BM4 G/G/NE G/G/NEG/NE/NE
  • Genomic DNA samples from leukemic or normal cells were examined for the presence of MLL gene rearrangements by Southern blot analysis, as described in Materials and Methods.

  • Abbreviations: R, rearranged; G, germline; NE, not examined; std, standard.