Table 2.

FACscan Analysis of Immunofluorescence Assays Performed on PBMC and Cell Subsets Purified From HCV-Infected Patients

Antibodies*CD Tested Cell-151Percent Detection
Before Selection After Selection Using Antibodies Directed Against*:
Total EventsCD45+-152CD3 CD15 CD19 CD14Neg Fraction-153
M833 CD45RB PBMC 62.3 100100 100 95-155 100 38¶
Leu4CD3 T lymphocytes 35.3 59.3 99 3 <1<1 37
Leu12 CD19 B lymphocytes 6.9 11.6<1 <1 94 <1 <1
LeuM1 CD15Granulocytes 20.5 24.9 <1 96 <1 <1<1
LeuM3 CD14 Monocytes 8.2 13.8 <1<1 <1 79 <1
CD42a Platelets 9.5x <1 <1 <1 <1 52
  • *Immunomagnetic selection and antibodies as described in Materials and Methods, 10,000 events were counted and analyzed for each antibody.

  • F0-151 Subset of cells expressing the corresponding CD.

  • F0-152 Percentage of fluorescent cells when CD45+ cells = 100%.

  • F0-153 Negative fraction collected at the end of the last immunomagnetic selection.

  • F0-155 The CD45 cells were dead cells (<5%) and erythrocytes (23% of the events).

  • ¶The CD45 cells were dead cells.