Table 6.

CFC in Week 5 Long-Term Cultures of AML Samples Under Various Growth Conditions

PatientTissueCFC per 106 CellsFold Change From HMF (% abnormal*)
(% abnormal*)Sl/SlSl/Sl JIL3 + SFSl/Sl + FLSl/Sl JIL3 + FLMixedMixed + FL
Day 0HMF
5 wk
1BM165 (40)53 (25)2 (6)3 (7)6-152
2BM30 (33)9 (25)2 (0)6-152 6 (21)
3BMND13 (53)ND8 (34)
3PB3,150 (90)15 (32)5 (60)4 (68)30 (5)6-152 12 (0)6-152
46-151 PB485 (73)34 (88)0.4 (85)2 (87)1 (11)6-152 2 (23)6-152
55 (71)1 (43)1 (52)0.8 (3)6-152 0.7 (15)6-152
5PB48,100 (100)307 (100)0.02 (92)0.8 (100)2 (100)0.6 (88)
6PB158 (87)2,790 (80)0.7 (82)0.5 (69)0.6 (82)0.4 (82)
7PB31,260 (95)129 (0)0.03 (29)0.7 (60)0.4 (68)1 (78)0.3 (52)4 (50)
8PB45 (67)6 (65)0.5 (44)3 (33)3 (35)16 (29)6-152 3 (28)6-152 59 (38)
10PB225 (50)3 (21)2 (0)25 (4)185 (13)547 (0)6-152 11 (0)6-152 429 (8)
  • Growth conditions as shown on Table 2.

  • F6-150 % Abnormal, % cytogenetically abnormal colonies detected by FISH.

  • F6-151 Data for two separate experiments in LTC.

  • F6-152 The difference in the proportion of cytogenetically abnormal CFC between this LTC condition and that of LTC containing HMF achieved a P value <.05 by chi-square analysis.