Table 1

Phenotype of mice transplanted with unfractionated BM or FACS-purified LSK cells

Unfractionated BMLSK cells
ControlSCLtTA/BCR-ABLControlSCLtTA/BCR-ABL
Total no. of transplanted cells per mouse1 × 1061 × 1061200 (+1 × 105 wt)1200 (+1 × 105 wt)
No. of transplanted LSK cells per mouse23039812001200
WBCs, ×109/L5.2 ± 0.0918.8 ± 9.9*4.82 ± 1.25.88 ± 1.9
Gr-1+CD11b+ cells in the PB, percentage20 ± 6.168.8 ± 14.52*28.5 ± 14.853.6 ± 16.1
Absolute no. of Gr-1+CD11b+ cells in the PB, ×103/μL0.9 ± 0.312.7 ± 7.4*1.5 ± 1.13.4 ± 2.1
Moribund appearance after BM transplantation, wkNever8Never11
  • WBC and Gr-1+CD11b+ cells were assessed 7 weeks after transplantation.

  • * P < .05 vs control group.

  • P < .05 vs ufBM SCLtTA/BCR-ABL group. ufBM, n = 5 (stg) or 4 (dtg); LSK, n = 4 each.