Table 1

Relative expression of EVI1 splice variants (-1D, -1A, -1B, -1C, -3L) and MDS1/EVI1 (ME), a priori karyotype and final FISH results in EVI1+ patients from cohorts A and B

No.1D1A1B1C3LMEKaryotype (ISCN 1995)FISH
Cohort A
    18501634199808190,145,XY,inv(3)(p12p2?4),−7[16]/46,XY[8]inv(3)
    2540215975731570,245,XY,t(3;3)(q21;q26),−7[20]ND
    325414734264790,146,XY,t(3;3)(q21;q26)[51]ND
    426514056758760,045,XX,−7[24]inv(3)
    535288334650,547,XX,del(5)(q23q34),+del(21)(q21q22)[4]/47,idem,t(2;3)(p2?2;q2?7)[20]/46,XX[3]t(2;3)
    6796943160225131345,XY,inv(3)(q21q26.2),−7[13]ND
    7687447543845,XY,−7[16]inv(3)
    8166177425432546,XY[14]NA
    950842131378948,XY,+9,+21[9]/49,idem,+21[14]NA
    10686408209573619547,XX,del(3)(q25) or del(3)(q21q26),+marNA
    115416350121214546,XX,t(6;11)(q27;q23)[36]NN
    12217174731032722453,XY,+6,+8,+9,t(11;16)(q23;p13),+13,+14,+19,+21[15]NN
    1322769488385526946,XXNA
    1412038085652728946,XX,t(6;11)(q27;q23)[28]NN
    1594494119468043746,XY[30]NA
    1618310131363062893447,XY,+?8(9%)/46,XYNA
    17543252185104846,XY,t(3;21)(q26;q22),del(12)(p12p13)[20]ND
    181313270,0210,246,XY[38]inv(3)
    192189110,5902446,XY,?der(11)(q2?)[3]/46,XY[18]NN
    201467160,5213246,XY,inv(3)(q21q26)[32]inv(3)
    2126992711358646,XY,t(11;19)(q23;p13.1)[15]/46,XY[5]NN
    227279277216NANA
Cohort B
    2316241256363598341945,XX,inv(3)(q22q26),−7[29]/46,XX[1]ND
    245844171167437445,XY,inv(3)(q22q26),−7[25]ND
    25506845119373500,245,XY,inv(3)(q12q26.2),−7[20]ND
    267001121161762440,045,XX,−7[27]/46,XX[3]inv(3)
    2721410744495410,1NANN
    281731722810460,046,XX[68]inv(3)
    291962396961670,046,XX,t(1;6)(p32;q24∼25),del(2)(q34)[33]/46,XX[1]inv(3)
    3082912581326833363NANA
    31745235414723130928645,XY,−7,t(9;11)(p21;q23)[33]NN
    323156411749253253246,XX,t(11;19)(q23;p13)[21]NN
    33207186118537316846,XY,t(9;22)(q34;q11)[22]NN
    341928971128326653946,XX,t(2;9;11)(p13;p22;q23)[20]NA
    358330643351744846,XY[40]NN
    367967363204106546,XX,del(7)(q22)[41]/46,XX[1]NN
    3752157270,960542946,XY,t(6;11)(q25;q23)[22]NN
    3814760,19513947,XY,+13[11]/46,XY[28]NN
    39163990,93322345,XY,−7,t(7;8)(q22;p11)[21]NN
    4011661,714439747,XX,+13[27]/46,XX[1]NN
    41141352,8234183046,XY,−7,add(12)(p12),+mar[46]/46,XY[54]NN
  • Relative expression of each of the distinct EVI1 transcripts was determined as explained in “Real-time quantitative PCR and Northern blot analysis.” A positive EVI1 or ME signal (ie, >30) is indicated by italics.

  • ME indicates MDS1/EVI1; ISCN, International System for Human Cytogenetic Nomenclature; FISH, fluorescent in situ hybridization; NN, normal 3q21, 3q26, and 3q telomere locus; inv(3), inversion of chromosomal band 3q21 and 3q26; NA, not available; NN, normal at the 3q21, 3q26, and 3q telomere locus as determined by FISH; and ND, not determined.