Table 2

RT-PCR primers and probes

Primer sequencesTm; MgCl2; product size, bp
UL13855°C; 1.5 mM; 333
    5′-TGC GCA TGT TTC TGA GCT AC-3′
    5′-ACG GGT TTC AAC AGA TCG AC-3′
UL138 DNA probe55°C; 1.5 mM; 150
    5′-GAG CTG TAC GGG GAG TAC GA-3′
    5′-AGC TGC ACT GGG AAG ACA CT-3′
UL122/12355°C; 2.5 mM; 194
    5′-ATT CTT CGG CCA ACT CTG GA-3′
    5′-ACA CGA TGG AGT CCT CTG CC-3′
UL122/123 DNA probe55°C; 2 mM; 201
    5′-CCC TGA TAA TCC TGA CGA GG-3′
    5′-CAT AGT CTG CAG GAA CGT CGT-3′
GAPDH60°C; 3 mM; 238
    5′-GAG TCA ACG GAT TTG GTC GT-3′
    5′-TTG ATT TTG GAG GGA TCT CG-3′
  • Primers are paired, with one forward and one reverse for each.

  • Tm indicates temperature.