Table 2

Correlation between complement regulatory protein expression and susceptibility to c1F6-mediated CDC

Cell linesComplement regulatory receptors*c1F6-mediated CDC (no. experiments)
CD46CD55CD59
IM-9++++++5 ± 1 (4)
Raji+++7 ± 0 (2)
Farage++++++0 ± 0 (3)
WIL2-S++56 ± 4 (6)
MHH-PREB-1++73 ± 5 (9)
L-428+++++++1 (1)
U266+++23 (1)
LP-1++64 ± 4 (4)
786-0+++++++0 (1)
Caki-1+++++++0 (1)
  • −indicates ratio less than 2; +, 2 to 9; ++, 10 to 49; and +++, at least 50.

  • * Complement regulatory protein (CRP) expression described as ratio of mean fluorescence intensity binding of FITC-anti-CRP antibody to background fluorescence from isotype control antibody.

  • Percent maximal cell lysis mediated by saturating concentrations of c1F6 in the presence of normal human serum. Mean ± standard error.

  • Single experiment with triplicate samples.