Table 1.

PCR primers and probes

GenesSequencesSizes, bp
SARS-CoV (F) 5′ TAC ACA CCT CAG CGT TG 3′ 182
(R) 5′ CAC GAA CGT GAC GAA T 3′
β-actin (F) 5′ CCC AAG GCC AAC CGC GAG AAG AT 3′ 219
(R) 5′ GTC CCG GCC AGC CAG GTC CAG 3′
IFN-α (F) 5′ CCT TCC TCC TGT CTG ATG GA 3′ 67
(P) 5′ (FAM) CAG ACA TGA CTT TGG ATT TCC CCA GG (TARMA) 3′
(R) 5′ ACT GGT TGC CAT CAA ACT CC 3′
IFN-β (F) 5′ GCC GCA TTG ACC ATC T 3′ 261
(R) 5′ AGG AGT ACA GTC ACT GTG 3′
IFN-γ (F) 5′ CTA ATT ATT CGG TAA CTG ACT TGA 3′ 75
(P) 5′ (FAM) TCC AAC GCA AAG CAA TAC ATG AAC (TARMA) 3′
(R) 5′ ACA GTT CAG CCA TCA CTT GGA 3′
TNF-α (F) 5′ GGC TCC AGG CGG TGC TTG TTC 3′ 409
(R) 5′ AGA CGG CGA TGC GGC TGA TG 3′
IL-6 (F) 5′ ATT CGG TAC ATC CTC GAC 3′ 331
(R) 5′ GGG GTG GTT ATT GCA TC 3′
IL-12 (F) 5′ CGG TCA TCT GCC GCA AA 3′ 80
(R) 5′ TGC CCA TTC GCT CCA AGA 3′
MIP-1α (F) 5′ CTC TGC ACC ATG GCT CTC TGC AAC 3′ 98
(R) 5′ TGT GGA ATC TGC CGG GAG GTG TAG 3′
RANTES (F) 5′ CCC CTC ACT ATC CTA CC 3′ 285
(R) 5′ TCA CGC CAT TCT CCT G 3′
IP-10 (F) 5′ CTG ACT CTA AGT GGC ATT 3′ 208
(R) 5′ TGA TGG CCT TCG ATT CTG 3′
MCP-1 (F) 5′ CAT TGT GGC CAA GGA GAT CTG 3′ 91
(R) 5′ CTT CGG AGT TTG GGT TTG CTT 3′
ACE2 (F) 5′ CTG GGA TCA GAG ATC GGA AGA 3′ 288
(R) 5′ CTC TCT CCT TGG CCA TGT TGT 3′
  • (F) indicates forward primers; (R), reverse primers; (P), Taqman probes; TNF, tumor necrosis factor; IFN, interferon; IL, interleukin; MIP, macrophage inflammatory protein; RANTES, regulated on activation, normal T cell expressed and secreted; IP-10, interferon-γ induced protein 10; MCP-1, monocyte chemoattractant protein 1, ACE2, angiotensin converting enzyme 2.