Table 1.

Nomenclature of parental and transduced Jurkat cell lines

Parental cellsIntroduced geneNomenclatureMean CD3 expression ± standard deviation*
P116 ZAP-/Syk- 440 ± 79
P116 ZAP ZAPE/Syk- 401 ± 84
P116 Syk ZAP-/SykE 392 ± 54
E6.1 Syk ZAP+/SykE 462 ± 91
77-6.8 ZAP+/Syk+ 234 ± 64
  • + indicates endogenous expression; E, ectopic expression; and —, no expression.

  • * Mean fluorescence intensity of CD3 expression as monitored by flow cytometry using an αCD3 mAb, mean ± standard deviation in 5 independent experiments.