Table 1.

Antibodies used for immunohistochemical stains

AntibodyCloneSourceAntigen retrievalDilution
CD20 L26 Dako, Carpinteria, CA Citrate 30 1:200
CD10 56C6 Ventana, Tucson, AZ Citrate 60 1:1
Bcl-6 Polyclonal Santa Cruz Biotechnology, Santa Cruz, CA EDTA 60 1:75
MUM1 MUM1p* Falini et al46 EDTA 30 1:10
Bcl-2 124 Dako EDTA 30 1:10
Cyclin D2 Polyclonal Santa Cruz Biotechnology EDTA 30 1:500
FOXP1 JC12 Banham et al66 EDTA 30 1:80
  • Citrate 30 indicates 30 minutes at 95°C in citrate (10 mM, pH 6.0); citrate 60 indicates 60 minutes at 95°C in citrate (10 mM, pH 6.0); EDTA 30 indicates 30 minutes at 95°C in EDTA (ethylenediaminetetraacetic acid; 1 mM, pH 8.0), EDTA 60 indicates 60 minutes at 95°C in EDTA (1 mM, pH 8.0).

  • * This clone is now commercially available from Dako.