Table 2.

Expression levels in ES cells infected with Pim-1 RNAi versus Flk1+/+ ES cells at 5.5 and 8.5 days of differentiation in vitro

Fold increase in Pim-1 RNAi
GeneDay 5.5Day 8.5
Flk-1 −2.7 ± 0.4 −14.2 ± 21
PECAM1 −2.8 ± 0.3 −4.5 ± 0.5
VE-cadherin −8.8 ± 1.2 −42 ± 6.3
Tie-2 −2.9 ± 0.3 −35.0 ± 4.4
Flt4 −5.9 ± 1.0 −1.4 ± 0.1
VEGF-A −12.3 ± 1.1 −1.3 ± 0.1
VEGF-D −1.6 ± 0.2 −2.5 ± 0.4
α-SMA −4.6 ± 0.6 −2.5 ± 0.3
SM22α −4.5 ± 0.5 −2.6 ± 0.4
Calponin-h1 −7.3 ± 0.9 −1.7 ± 0.2
SM-MHC −3.7 ± 0.3 +1.7 ± 0.1
Brachyury −37.3 ± 4.9 +1.0 ± 0.1
α 1-fetoprotein −1.1 ± 0.1 +143 ± 18
Nestin +1.4 ± 0.2 −3.5 ± 0.5
Myogenin +81.2 ± 11 +129.0 ± 15
  • Data are presented as mean ± SEM. The fold change detected by quantitative RT-PCR represents the average among the results of 4 independent measurements.