Table 1.

Differentiation of 32Dcl3 cells is blocked by expression of FLT3/ITD

CellsDay 0Day 9
32D/pBabe32D/FLT332D/FLT3/ITD32D/pBabe32D/FLT332DFLT3/ITD
Myeloblasts, %81.3 ± 2.585.7 ± 3.882.3 ± 4.514.3 ± 2.511.3 ± 3.285.3 ± 5.0
Intermediates, %18.7 ± 2.514.3 ± 3.817.7 ± 4.564 ± 3.666.7 ± 4.514.7 ± 5.0
Granulocytes, %00 0 21.7 ± 4.014.7 ± 5.00
  • Differential counts of 32D/pBabe, 32D/FLT3, and 32D/FLT3/ITD cell cultures induced by G-CSF (20 ng/mL) were performed on days 0 and 9.

  • Values are the mean percentages, ± SD, of cells from 3 independent counts examined by light microscopy after Wright-Giemsa staining of the cytospins. “Intermediates” include promyelocytes, myelocytes, and metamyelocytes.