Table 1

Quantification of Munc13-4 in mouse platelets

Wild-typeUnc13dJinx Het
ng/5 × 107 platelets*Molecules/plateletng/5 × 107 platelets*Molecules/platelet
Munc13-43.31 ± 0.50317.6 ± 47.62.43 ± 0.15233 ± 14.8
VAMP-88.36 ± 0.118360 ± 110NDND
Syntaxin 24.87 ± 0.331766 ± 121NDND
Syntaxin 44.61 ± 0.521625 ± 184NDND
  • ND indicates not determined.

  • * Mass of proteins in nanograms (ng) per 5 × 107 platelets was determined by quantitative Western blotting using recombinant proteins to generate a standard curve.

  • Number of molecules per platelet was calculated using the molecular weight for Munc13-4 (125 kDa), VAMP-8 (12 kDa), syntaxin 2 (33 kDa), and syntaxin 4 (34 kDa).

  • From Graham et al.3