Making HSCs in vitro: don't forget the hemogenic endothelium

Bradley W. Blaser and Leonard I. Zon


Generating an HSC in vitro from non-hematopoietic tissue has been a goal of experimental hematologists for decades. Until recently, no in vitro-derived cell has closely demonstrated the full lineage potential and self-renewal capacity of a true HSC. Studies revealing stem cell ontogeny from embryonic mesoderm to hemogenic endothelium to HSC provided the key to inducing HSC-like cells in vitro from a variety of cell types. Here we review the path to this discovery and discuss the future of autologous transplantation with in vitro-derived HSCs as a therapeutic modality.

  • Submitted April 4, 2018.
  • Accepted August 4, 2018.