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Cooperative PSGL-1 and CXCR2 signaling in neutrophils promotes deep vein thrombosis in mice

Tadayuki Yago, Zhenghui Liu, Jasimuddin Ahamed and Rodger P. McEver

Key points

  • Cooperative PSGL-1 and CXCR2 signaling in neutrophils increases adhesion and NET release in flow-restricted veins.

  • Cooperative PSGL-1 and CXCR2 signaling in neutrophils, but not monocytes, promotes deep vein thrombosis.

Abstract

Inflammation is a major contributor to deep vein thrombosis (DVT). Flow restriction of the inferior vena cava (IVC) in mice induces DVT like that in humans. In this model, P-selectin-dependent adhesion of neutrophils and monocytes leads to release of neutrophil extracellular traps (NETs) and expression of tissue factor. However, it is not known what signals cause myeloid cells to generate these procoagulant effectors. Using ultrasonography and spinning-disk intravital microscopy in genetically engineered mice, we found that engagement of P-selectin glycoprotein ligand-1 (PSGL-1) and the chemokine receptor CXCR2 on rolling neutrophils propagated signals that cooperated to induce β2 integrin-dependent arrest in flow-restricted IVCs. Unlike previous reports, PSGL-1 signaling in neutrophils did not require L-selectin, and it employed tyrosine 145 rather than tyrosines 112 and 128 on the adaptor Src homology domain-containing leukocyte phosphoprotein of 76 kDa (SLP-76). PSGL-1 and CXCR2 signaling cooperated to increase the frequency and size of thrombi, in part by stimulating release of NETs. Unlike in neutrophils, blocking PSGL-1 or CXCR2 signaling in monocytes did not affect their recruitment into thrombi or their expression of tissue factor. Our results demonstrate that neutrophils cooperatively signal through PSGL-1 and CXCR2 to promote DVT.

  • Submitted May 11, 2018.
  • Accepted July 26, 2018.