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Oxidative modifications of extracellular matrix promote the second wave of inflammation via β2 integrins

Valentin P. Yakubenko, Kui Cui, Christopher L. Ardell, Kathleen E. Brown, Xiaoxia Z. West, Detao Gao, Samantha Stefl, Robert G. Salomon, Eugene A. Podrez and Tatiana V. Byzova

Key points

  • Recruitment of neutrophils generates phospholipid oxidation and formation of carboxyethylpyrrole (CEP) adducts with ECM proteins.

  • CEP-protein adducts form inflammatory-specific substrate for αMβ2 and αDβ2 integrin-mediated macrophage migration during inflammation.

Abstract

Early stages of inflammation are characterized by extensive oxidative insult by recruited and activated neutrophils. Secretion of peroxidases, including the main enzyme, myeloperoxidase, leads to the generation of reactive oxygen species. We show that this oxidative insult leads to polyunsaturated fatty acid, e.g. DHA, oxidation, and accumulation of its product 2-(ω-carboxyethyl)pyrrole (CEP), which, in turn, is capable of protein modifications. In vivo CEP is generated predominantly at the inflammatory sites in macrophage-rich areas. During thioglycollate-induced inflammation, neutralization of CEP adducts dramatically reduced macrophage accumulation in the inflamed peritoneal cavity while exhibiting no effect on the early recruitment of neutrophils, suggesting a role in the second wave of inflammation. CEP modifications were abundantly deposited along the path of neutrophils migrating through the 3D fibrin matrix in vitro. Neutrophil-mediated CEP formation was markedly inhibited by the myeloperoxidase inhibitor, 4-ABH, and significantly reduced in MPO-deficient mice. On macrophages, CEP adducts were recognized by cell adhesion receptors, integrin αMβ2 and αDβ2. Macrophage migration through CEP-fibrin gel was dramatically augmented when compared to fibrin alone, and was reduced by β2-integrin deficiency. Thus, neutrophil-mediated oxidation of abundant polyunsaturated fatty acids leads to the transformation of existing proteins into stronger adhesive ligands for αMβ2- and αDβ2–dependent macrophage migration. The presence of a carboxyl group rather than a pyrrole moiety on these adducts, resembling characteristics of bacterial and/or immobilized ligands, is critical for recognition by macrophages. Therefore, specific oxidation-dependent modification of extracellular matrix, aided by neutrophils, promotes subsequent αMβ2- and αDβ2-mediated migration/retention of macrophages during inflammation.

  • Submitted October 10, 2017.
  • Accepted April 19, 2018.