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Mature CD10+ and immature CD10- neutrophils present in G-CSF-treated donors display opposite effects on T cells

Olivia Marini, Sara Costa, Dalila Bevilacqua, Federica Calzetti, Nicola Tamassia, Cecilia Spina, Donata De Sabata, Elisa Tinazzi, Claudio Lunardi, Maria T. Scupoli, Chiara Cavallini, Elisa Zoratti, Ilaria Tinazzi, Antonio Marchetta, Aurora Vassanelli, Maurizio Cantini, Giorgio Gandini, Andrea Ruzzenente, Alfredo Guglielmi, Francesco Missale, William Vermi, Cristina Tecchio, Marco A. Cassatella and Patrizia Scapini

Key points

  • CD10 as a marker discriminating mature from immature neutrophils within heterogeneous neutrophil populations in pathological settings.

  • Immunosuppressive mature CD66b+CD10+ and immunostimulatory immature CD66b+CD10- neutrophils coexist in G-CSF-treated donors.

Abstract

The identification of discrete neutrophil populations, as well as the characterization of their immunoregulatory properties, is an emerging topic under extensive investigation. In such regard, the presence of circulating CD66b+-neutrophil populations, exerting either immunosuppressive or proinflammatory functions, has been described in several acute and chronic inflammatory conditions. However, due to the lack of specific markers, the precise phenotype and maturation status of these neutrophil populations remain unclear. Herein, we report that CD10, also known as common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), or enkephalinase, can be used as a marker that, within heterogeneous populations of circulating CD66b+-neutrophils present in inflammatory conditions, clearly distinguishes the mature from the immature ones. Accordingly, we observed that the previously described immunosuppressive neutrophil population that appears in the circulation of healthy volunteers receiving G-CSF for stem cell mobilization (GDs) consists of mature CD66b+CD10+-neutrophils displaying an activated phenotype. These neutrophils inhibit proliferation and IFNγ production by T cells via a CD18-mediated contact-dependent arginase 1 release. By contrast, we found that immature CD66b+CD10--neutrophils, also present in GDs, display an immature morphology, promote T-cell survival and enhance proliferation and IFNγ production by T cells. Altogether, our findings uncover that in GDs, circulating mature and immature neutrophils, distinguished by their differential CD10 expression, exert opposite immunoregulatory properties. Therefore, CD10 might be utilized as a phenotypic marker discriminating mature neutrophils from immature neutrophil populations present in patients with acute or chronic inflammatory conditions, as well as facilitating their isolation, to better define their specific immunoregulatory properties.

  • Submitted April 27, 2016.
  • Accepted December 19, 2016.