Blood Journal
Leading the way in experimental and clinical research in hematology

Bioengineered human vascular networks transplanted into secondary mice reconnect with the host vasculature and re-establish perfusion

  1. Kyu-Tae Kang1,
  2. Patrick Allen2, and
  3. Joyce Bischoff1,*
  1. 1 Vascular Biology Program and Department of Surgery, Children's Hospital Boston, Harvard Medical School, MA, United States;
  2. 2 Department of Biomedical Engineering, Boston University, MA, United States
  1. * Corresponding author; email: joyce.bischoff{at}


The ability to form anastomoses with the host circulation is essential for vascular networks incorporated within cell-seeded bioengineered tissues. Here, we tested whether and how rapidly human endothelial colony forming cell (ECFC)/mesenchymal progenitor cell (MPC)-derived bioengineered vessels, originally perfused in one mouse, could become re-perfused in a secondary mouse. Using in vivo labeling with a systemically injected mixture of human- and murine-specific lectins, we demonstrate that ECFC/MPC-blood vessels reconnect and are perfused at day 3 after transplantation. Furthermore, we quantified the longitudinal change in perfusion volume in the same implants before and after transplantation using contrast-enhanced micro-ultrasonic imaging. Perfusion was restored at day 3 after transplantation and increased with time, suggesting an important new feature of ECFC/MPC-blood vessels: the bioengineered vessels can reconnect with the vasculature when transplanted to a new site. This feature extends the potential applications of this postnatal progenitor cell-based technology for transplantable large tissue-engineered constructs.

  • Submitted August 30, 2011.
  • Accepted October 23, 2011.