Data supplements

  • Supplemental materials for: Hughes et al

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    • Figure S1. Immunohistochemical staining for IL-22 and CD117 in tissue sections from human tonsil (JPG, 62 KB) -
      Immunohistochemistry was performed as previously described1. Briefly, the Ultraview Universal system (DAB, CD117 and fast red, IL-22) was employed. The paraffin-embedded tonsillar tissue sections were digested in Protease 1 for 4 minutes and then incubated with the anti-human IL-22 (R&D systems, Minneapolis, MN) antibody (1:100). The slides were photographed at room temperature, the coverslip removed, and the slides then tested with the anti-human CD117 (1:1000, DakoCytomation, Glostrup, Denmark) after antigen retrieval for 30 minutes in CC1. Images were digitally acquired using the following equipment, all from Olympus (Olympus Imaging America Inc., Center Valley, PA): DP 12 camera, BX50 microscope, and UPLANF1 objectives at 400× magnification power. Images were analyzed using Adobe Photoshop CS3 software (Adobe Systems, Inc., San Jose, CA). The different colored arrows indicate the exact same cell staining for IL-22 (left) and co-labeling with IL-22 and CD117 (right) from serial sections of the same region. Cells with red staining are IL-22(+). Cells with brown staining are CD117(+). Staining indicated that CD117(+)IL-22(+) immature NK cells reside in the epithelia and lamina propria as well as the parafollicular T-cell�rich regions of SLT.