Blood Journal
Leading the way in experimental and clinical research in hematology

High frequency of t(12;21) in childhood B-lineage acute lymphoblastic leukemia

  1. SP Romana,
  2. H Poirel,
  3. M Leconiat,
  4. MA Flexor,
  5. M Mauchauffe,
  6. P Jonveaux,
  7. EA Macintyre,
  8. R Berger, and
  9. OA Bernard
  1. U301 de l'Institut National de la Sante et de la Recherche Medicale, Paris, France.

Abstract

The recurrent t(12;21)(p12;q22) translocation fuses two genes, TEL and AML1, that have previously been shown to be independently involved in myeloid malignant proliferations. A search for rearrangement of the TEL locus in the region known to be involved in t(12;21) was performed by Southern blotting in a panel of hematopoietic malignancies. The presence of a t(12;21) was confirmed by fluorescence in situ hybridization (FISH) and/or reverse transcriptase (RT)-polymerase chain reaction (PCR). We report that fusion of TEL to AML1 is specifically observed in at least 16% of the childhood B-lineage acute lymphoblastic leukemia (ALL) investigated, none of which had been previously identified as harboring t(12;21).