Blood Journal
Leading the way in experimental and clinical research in hematology

Inactivation of human immunodeficiency virus type 1 by ozone in vitro

  1. KH Wells,
  2. J Latino,
  3. J Gavalchin, and
  4. BJ Poiesz
  1. Department of Medicine, SUNY Health Science Center, Syracuse 13210.

Abstract

A device was designed to deliver a constant source of given concentrations of ozone to fluids containing human immunodeficiency virus type 1 (HIV-1). Ozone was found to inactivate HIV-1 virions in a dose-dependent manner. Greater than 11 log inactivation was achieved within 2 hours at a concentration of 1,200 ppm ozone. Similar concentrations of ozone had minimal effect on factor VIII activity in both plasma and immunoaffinity-purified preparations of factor VIII treated for the same time period. The data indicate that the antiviral effects of ozone include viral particle disruption, reverse transcriptase inactivation, and/or a perturbation of the ability of the virus to bind to its receptor on target cells. Ozone treatment offers promise as a means to inactivate human retroviruses in human body fluids and blood product preparations.