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Separation of Megakaryocytes From Mouse Bone Marrow by Velocity Sedimentation

A. Nakeff and B. Maat

Abstract

A technique for separating mouse bone marrow megakaryocytes by unit gravity velocity sedimentation is described using a modified sedimentation chamber. The velocity sedimentation profile for megakaryocytes comprises three peaks corresponding to sedimentation velocities of 30, 60, and 100 mm/hr. Morphologic examination of megakaryocytes in the 30 mm/hr peak reveals them to be primarily immature with the latter two peaks corresponding to larger, more mature megakaryocytes.

  • Submitted January 3, 1973.
  • Revision received August 17, 1973.
  • Accepted August 27, 1973.