Blood Journal
Leading the way in experimental and clinical research in hematology

Colony Formation by Normal and Leukemic Human Marrow Cells in Culture: Effect of Conditioned Medium From Human Leukocytes

  1. N. N. ISCOVE, M.D., Fellow of the Medical Research Council of Canada,
  2. J. S. SENN, M.D., F.R.C.P.(C), Head of Clinical Hematology,
  3. J. E. TILL, PH.D., and
  4. E. A. MCCULLOCH, M.D., F.R.C.P.(C), Institute of Medical Science
  1. Institute of Medical Science, University of Toronto, the Ontario Cancer Institute and Sunnybrook Hospital, Toronto, Canada.
  2. Institute of Medical Science, University of Toronto, Toronto, Canada.
  3. Sunnybrook Hospital, Toronto, Canada.
  4. Division of Biological Research, Ontario Cancer Institute, Toronto, Canada.
  5. University of Toronto and the Ontario Cancer Institute, Toronto, Canada.

Abstract

"Conditioned medium" obtained from cultures of human peripheral leukocytes promoted the growth of human marrow cells in cell culture. This material also permitted the growth of small colonies from the marrow of patients with acute myelogenous leukemia in relapse; in its absence, only occasional colonies were observed.

  • Submitted June 30, 1970.
  • Accepted July 24, 1970.