Blood Journal
Leading the way in experimental and clinical research in hematology

Normal and Variant Isoenzymes of Human Blood Cell Hexokinase and the Isoenzyme Patterns in Hemolytic Anemia

  1. CIGDEM ALTAY, Research Fellow in Pediatrics,
  2. CHESTER A. ALPER, Associate in Hematology, and
  3. DAVID G. NATHAN, Chief
  1. Hematology Division of the Department of Medicine of the Children’s Hospital Medical Center and the Blood Grouping Laboratory, Boston, Mass., and the Department of Pediatrics of the Harvard Medical School, Boston, Mass.
  2. Harvard Medical School Trainee in Hematology, Children's Hospital Medical Center, Boston, Mass.
  3. Children's Hospital Medical Center. Director of Serum Protein Research, Blood Grouping Lab., Boston Associate Professor of Pediatrics, Harvard Medical School, Cambridge, Mass.
  4. Division of Hematology, Children's Hospital Medical Center. Assistant Professor of Pediatrics, Harvard Medical School, Cambridge, Mass.

Abstract

Electrophoresis of red cell hexokinase in agarose electrophoresis revealed two major (1 and 2) and two minor (3 and 4) bands. Platelet and leukocyte hexokinase patterns differed from those of red cells. There was a strong band 1, but considerably faster bands termed 5, 6 and 7 were also observed which were sensitive to changes in glucose concentration. The presence of contaminating leukocytes can significantly alter the electrophoretic pattern of "red cell" hexokinase activity. Bands 2, 3 and 4 of red cells appeared to be synthesized independently of band 1 and absence of band 1 did not effect either red cell metabolism or survival. Absence of bands 2, 3 and 4 may be associated with hemolytic anemia, decreased erythrocyte hexokinase activity and decreased erythrocyte glycolysis. Young red cells had increased activity of all bands, particularly band 2. No influence of hemoglobin type on hexokinase patterns was observed, nor was there any selective influence of cell storage, medium glucose, or 2-mercaptoethanol on individual bands. None of the various isoenzyme patterns were associated with abnormal hexokinase kinetics.

  • Submitted September 26, 1969.
  • Revision received January 21, 1970.
  • Accepted January 23, 1970.