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CD207+CD1a+ cells circulate in pediatric patients with active Langerhans cell histiocytosis

Eugenio Antonio Carrera Silva, Wanda Nowak, Licina Tessone, Cinthia Mariel Olexen, Juan Manuel Ortiz Wilczyñski, Ivana Gisele Estecho, Graciela Elena, Andrea Emilse Errasti and Diego Alfredo Rosso

Key Points

  • CD207+CD1a+ cells are present in the circulation of patients with active LCH.

  • TGF-β and TSLP levels are increased in the plasma of patients with active LCH.

Publisher's Note: There is an Inside Blood Commentary on this article in this issue.

Abstract

Langerhans cell histiocytosis (LCH) is a rare disease with an unknown etiology characterized by heterogeneous lesions containing CD207+CD1a+ cells that can arise in almost any tissue and cause significant morbidity and mortality. Precursors of pathological Langerhans cells have yet to be defined. Our aim was to identify circulating CD207+CD1a+ cells and their inducers in LCH. Expression of CD207 and CD1a in the blood myeloid compartment as well as thymic stromal lymphopoietin (TSLP) and transforming growth factor β (TGF-β) plasma levels were measured in 22 pediatric patients with active disease (AD) or nonactive disease (NAD). In patients with AD vs those with NAD, the myeloid compartment showed an increased CD11b (CD11bhigh plus CD11b+) fraction (39.7 ± 3.6 vs 18.6 ± 1.9), a higher percentage of circulating CD11bhighCD11c+CD207+ cells (44.5 ± 11.3 vs 3.2 ± 0.5), and the presence of CD11chighCD207+CD1a+ cells (25.0 ± 9.1 vs 2.3 ± 0.5). Blood CD207+CD1a+ cells were not observed in adult controls or umbilical cord. Increased TSLP and TGF-β levels were detected in patients with AD. Interestingly, plasma from patients with AD induces CD207 expression on CD14+ monocytes. We conclude that CD207+CD1a+ cells are circulating in patients with active LCH, and TSLP and TGF-β are potential drivers of Langerhans-like cells in vivo.

  • Submitted May 3, 2017.
  • Accepted August 16, 2017.
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