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Mechanisms of platelet-mediated liver regeneration

Ton Lisman and Robert J. Porte

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  • RE: Ethylenediaminetetraacetic acid (EDTA) Plasma Preparation Results in In Vitro Platelet Activation and is Therefore Not Suitable to Monitor In Vivo Release of Platelet-derived Growth Factors
    • Patrick Starlinger, Surgical Oncologist Medical University of Vienna, Department of Surgery
    • Other Contributors:
      • Alice Assinger, Scientist

    We read with great interest the recent Blood Spotlight by Lisman et al. 1 Within this review as well as in a recent report the authors refer to their own data on soluble circulating platelet-derived growth factors. 1,2 Their results, as pointed out in this Blood Spotlight, are in part contradictory to our previous reports.3,4 During the past few years we optimized and validated plasma preparation protocols to avoid in vitro platelet activation. 3-5 We ended up in routinely performing citrate, theophylline, adenosine, dipyridamole (CTAD) plasma preparation at 4°C, immediately processed, to measure in vivo circulating platelet-derived growth factor levels. 3-5 Within our initial analysis we also evaluated ethylenediaminetetraacetic acid (EDTA) plasma preparation, as performed in the reports by Lisman and coworkers.2,6 Unfortunately, as illustrated in Figure 1, EDTA preparation at room temperature is associated with a substantial degree of in vitro platelet granule release, resulting in artificially elevated platelet-derived growth factor levels. Indeed, the levels of platelet-derived growth factors within one of the recent manuscripts using this plasma preparation method, are substantially higher than reported by us and closely equal the artificially increased levels using EDTA plasma preparation (illustrated in Figure 1).2 Accordingly, small biologically relevant changes of circulating platelet-derived growth factors cannot be detected with this plasma preparation method....

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    Conflict of Interest:
    None declared.