Advertisement

A genome editing primer for the hematologist

Megan D. Hoban and Daniel E. Bauer

Published e-Letters

Compose eLetter

Plain text

  • No HTML tags allowed.
  • Web page addresses and e-mail addresses turn into links automatically.
  • Lines and paragraphs break automatically.
Author Information
First or given name, e.g. 'Peter'.
Your last, or family, name, e.g. 'MacMoody'.
Your email address, e.g. higgs-boson@gmail.com
Your role and/or occupation, e.g. 'Orthopedic Surgeon'.
Your organization or institution (if applicable), e.g. 'Royal Free Hospital'.
Statement of Competing Interests
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Image CAPTCHA
Enter the characters shown in the image.

Vertical Tabs

Jump to comment:

  • RE: A Genome Editing Primer For The Hematologist
    • Prateek Bhatia, Pediatric Hematologist Post Graduate Institute Of Medical Education and Research
    • Other Contributors:
      • Aditya Singh, PhD Scholar Pediatric Hematology-Oncology

    We read this very interesting article by Megan D. Hoban and Daniel E. Bauer1 that basically outlines current usage and future prospects of various genome editing tools for a clinical and experimental hematologist. The article is very well written and interests us since we are currently working with CRISPR-Cas9 tool to truncate BCR-ABL carcinogenic chimeric protein products p210 and p190 of CML and ALL respectively. The use of this tool in editing a chimeric transcript is currently unexplored and poses few challenging issues. We are working on targeting CRISPR specifically at the chimeric region so as it has minimal; ideally zero off-target effects. While designing the system (see figure 1), we used various available servers online, including the one developed by Chang et al2, however all of them lacked the feature of finding sgRNAs of chimeric region which could guide us by choosing one with highest score and optimal span in the two genes so that it does not attach to wild type of any of those genes as off target.
    The authors have rightfully raised the question regarding the need to increase homology directed repair (HDR) efficiency but have missed on citing a few researches that we think are worthy of discussion3-5. Especially, Richardson et al3 have pointed out a very interesting finding that HDR can be enhanced by 60% if the template is designed complementary to the sgRNA with 36 bp on the protospacer adjacent motif (PAM) distal side and 91 bp extensions on PAM p...

    Show More

    Attachment(s)

    Conflict of Interest:
    None declared.