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Characteristic repartition of monocyte subsets as a diagnostic signature of chronic myelomonocytic leukemia

Dorothée Selimoglu-Buet, Orianne Wagner-Ballon, Véronique Saada, Valérie Bardet, Raphaël Itzykson, Laura Bencheikh, Margot Morabito, Elisabeth Met, Camille Debord, Emmanuel Benayoun, Anne-Marie Nloga, Pierre Fenaux, Thorsten Braun, Christophe Willekens, Bruno Quesnel, Lionel Adès, Michaela Fontenay, Philippe Rameau, Nathalie Droin, Serge Koscielny and Eric Solary on behalf of the Groupe Francophone des Myélodysplasies

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  • RE: CD16neg monocytes obtained on a routine complete blood count as a CMML diagnostic marker
    • Cedric PASTORET, Clinical Biologist specialized in Cellular Hematology CHU de Rennes, Pole Biologie, Rennes, France
    • Other Contributors:
      • Marc LE MOUEL, Resident in Clinical Biology
      • Tony MARCHAND, MD/Clinical Hematology
      • Thierry FEST, MD/PhD/Clinical Biologist specialized in Cellular Hematology
      • Mikael ROUSSEL, MD/PhD/Clinical Biologist specialized in Cellular Hematology

    Cedric Pastoret,1 Marc Le Mouel,1 Tony Marchand,2 Thierry Fest1 and Mikael Roussel1

    Laboratory of Hematology, University Hospital of Rennes

    1 CHU de Rennes, Pôle Biologie, Rennes, France
    2 CHU de Rennes, Service d'Hématologie Clinique, Rennes, France

     

    We read with interest the article by Selimoglu-Buet and colleagues validating the fraction of CD16neg-monocytes (%CD16negMo) as a diagnostic criteria in chronic myelomonocytic leukemia (CMML).1 In our institution, we implemented the Hematoflow (HF) (Beckman Coulter, Brea, CA) as part of the complete blood count (CBC) workflow.2,3 The HF uses antibodies, including CD36 and CD16, to report a 5-part differential and cell subsets such as CD16pos- and CD16neg- monocyte. Here, we would like to report our experience in identifying CMML patients with the %CD16negMo, obtained on the basis of a regular CBC.

    We identified retrospectively, over a period of 4 years, 42 CMML patients with a HF at diagnosis. We set up a control group with 95 patients with monocytosis (>1x109/L) lacking CMML criteria. The mean count of monocyte was at 5.42x109/L (95% confidence interval (CI) 2.42-8.42) and at 2.21x109/L (95%CI 2.08-2.33) for CMML and control groups, respectively. We found an optimal %CD1...

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    Conflict of Interest:
    None declared.