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Sethi G, Ahn KS, Pandey MK, Aggarwal BB. Celastrol, a novel triterpene, potentiates TNF-induced apoptosis and suppresses invasion of tumor cells by inhibiting NF-κB-regulated gene products and TAK1-mediated NF-κB activation. Blood. 2007;109(7):2727-2735.

On page 2730 in the 1 April 2007 issue, there are errors in Figure 2B and 2C. The β-actin loading controls in panels B and C were from the same experiment, and the membrane was stripped and reprobed for the proteins shown in the 2 panels; therefore, the β-actin loading controls are from the same blot for both panels. However, the TNF and celastrol + TNF lanes were erroneously duplicated between the left and right sides of both panels B and C. This has now been corrected, with substitution of the appropriate actin lanes in the revised panels B and C shown in the corrected Figure 2.

Figure 2

Celastrol inhibits TNF-induced NF-κB–regulated gene products. (A) Celastrol inhibits the expression of antiapoptotic gene products such as survivin, IAP1, IAP2, Bcl-XL, Bcl-2, and c-FLIP. KBM-5 cells (2 × 106 /mL) were left untreated or were incubated with 2.5 μM celastrol for 6 hours and then treated with 1 nM TNF for different amounts of time. Whole-cell extracts were prepared, and 30 μg of the whole-cell lysate was analyzed by Western blotting using antibodies against IAP1, IAP2, Bcl-xl, Bcl-2, cFLIP, and survivin as indicated. (B) Celastrol inhibits COX-2 and cyclin D1 expression induced by TNF. KBM-5 cells (2 × 106/mL) were left untreated or were incubated with 2.5 μM celastrol for 6 hours and then treated with 1 nM TNF for different times. Whole-cell extracts were prepared, and 30 μg of the whole-cell lysate was analyzed by Western blot analysis using antibodies against COX-2 and cyclin D1. (C) Celastrol inhibits VEGF and MMP-9 expression induced by TNF. KBM-5 cells (2 × 106 mL) were left untreated or were incubated with 2.5 μM celastrol for 6 hours and then treated with 1 nM TNF for different times. Whole-cell extracts were prepared, and 30 μg of the whole-cell lysate was analyzed by Western blot analysis using antibodies against MMP-9 and VEGF. Data are for a representative experiment of 3 independent experiments showing similar results.