Blood Journal
Leading the way in experimental and clinical research in hematology

Review Series: Epigenetics in Hematology
The role of chromatin modifiers in normal and malignant hematopoiesis

  1. Jill S. Butler1,2 and
  2. Sharon Y. R. Dent1,2,3
  1. 1Department of Molecular Carcinogenesis, The Virginia Harris Cockrell Cancer Research Center, The University of Texas MD Anderson Cancer Center Science Park, Smithville, TX;
  2. 2Center for Cancer Epigenetics, and
  3. 3The Genes & Development Program, Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX

Abstract

Complex developmental processes such as hematopoiesis require a series of precise and coordinated changes in cellular identity to ensure blood homeostasis. Epigenetic mechanisms help drive changes in gene expression that accompany the transition from hematopoietic stem cells to terminally differentiated blood cells. Genome-wide profiling technologies now provide valuable glimpses of epigenetic changes that occur during normal hematopoiesis, and genetic mouse models developed to investigate the in vivo functions of chromatin-modifying enzymes clearly demonstrate significant roles for these enzymes during embryonic and adult hematopoiesis. Here, we will review the basic science aspects of chromatin modifications and the enzymes that add, remove, and interpret these epigenetic marks. This overview will provide a framework for understanding the roles that these molecules play during normal hematopoiesis. Moreover, many chromatin-modifying enzymes are involved in hematologic malignancies, underscoring the importance of establishing and maintaining appropriate chromatin modification patterns to normal hematology.

Chromatin structure and epigenetic mechanisms

Hematopoiesis is an orderly process that involves the coordination of stem cell self-renewal, controlled expansion of progenitor cells, and timely differentiation. Developmental programs, such as hematopoiesis, are orchestrated by changes in patterns of gene expression. These patterns are directed by transcription factors, and the actions of these factors are strongly influenced by the chromatin structures of their target genes.

The basic repeating unit of chromatin is the nucleosome, which contains 2 copies of each core histone protein, H3, H4, H2A, and H2B and 146 base pairs of DNA spooled around the histone octamer. Histone H3 and histone H4 form a heterotetramer at the heart of the nucleosome, and 2 heterodimers of histones H2A and H2B associate with the tetramer to complete the octamer. Linker histones, typified by H1, bind to DNA entering into and exiting from the nucleosome, thereby providing further structural stability.

Chromatin that is in an open configuration, accessible to transcription factors and RNA polymerase, is often referred to as euchromatin, reflecting a less-condensed appearance in microscopic images of nuclear sections. Heterochromatin, in contrast, refers to more densely packed structures that are generally transcriptionally silent. Regions of the genome that include telomeres and centromeres are highly heterochromatic. Chromatin organization in other regions of the genome is more dynamic, reflecting changes in transcriptional competence and activity. Such plasticity is essential for regulation of various cellular processes such as cell division, gene transcription, DNA repair, replication, and recombination.

The term epigenetics is defined as inherited variation that occurs without changes in the DNA sequence.1 Epigenetic mechanisms allow a level of plasticity for the genetic information encoded by the DNA, which allows for the establishment of cell-specific expression programs. Modifications added to or removed from the chromatin template that carry epigenetic information include DNA methylation and histone posttranslational modifications (PTMs). Noncoding RNA molecules are also emerging as important regulators of gene expression during hematopoiesis.2,3 However, this review will highlight examples of the essential roles that chromatin-modifying enzymes play during hematopoietic cell development by regulating the dynamic nature of DNA and specific histone modifications, namely methylation and acetylation.

DNA modifications

Methylation of a cytosine residue in the context of a CpG dinucleotide represents a stable regulatory mark in the mammalian genome. Cytosine methylation (5-methyl cytosine [5mC]) is generally associated with heterochromatin formation and transcriptional repression. Conversely, stretches of unmethylated CpG dinucleotides, termed CpG islands, are often found in the promoters of actively transcribed genes and support a euchromatic environment. Additional functional groups were recently discovered to modify the 5-position of cytosine residues, namely hydroxymethyl (5hmC), formyl (5fC), and carboxyl (5caC), and the biological functions of these modifications throughout the genome are now being discovered.4

Covalent PTM of histone proteins

PTMs occurring on histone proteins regulate chromatin structure in a temporal and spatial manner. Histone modifications serve both as signaling mechanisms and as binding platforms to recruit other proteins. Covalent PTMs added to the core histone proteins include acetylation, methylation, phosphorylation, ubiquitination, sumoylation, adenosine 5'-diphosphate ribosylation, and biotinylation. The “writers” and “erasers” that govern the addition or removal of these PTMs often demonstrate site specificity; however, multiple enzymes are capable of modifying the same residue within a histone substrate.

Different patterns of histone PTMs have been proposed to act as a “code” in that specific patterns confer specific biological responses.5,6 The “histone code” hypothesis requires a way for effector molecules to interpret the information given by histone PTMs to mediate cellular processes outside of chromatin. Protein domains that recognize individual or combinatorial histone modifications have been termed reading or presenting domains.7 As more histone PTM reading domains are discovered, linking binding patterns to associated biological responses is becoming more complex. Accumulating evidence links deregulation of histone PTM interpretation with oncogenic transformation, highlighting the importance of histone reader proteins.8

Lysine acetylation and methylation occur on all 4 of the core histones, most prevalently on the N-terminal tails. The addition of an acetyl group neutralizes the positive charge of the lysine residue, which weakens interactions between the histones and DNA, facilitating more open and relaxed chromatin states. Acetylated histones are generally associated with transcriptionally active genes and euchromatic regions of the genome9 (Figure 1A). Lysine residues can accommodate up to 3 methyl groups (mono-, di-, or tri-), whereas arginine residues may be monomethylated or dimethylated, yielding monomethyl arginine, asymmetric dimethyl arginine, or symmetric dimethyl arginine. The degree of methylation at a particular lysine or arginine residue is just as important in influencing biological outcome as the target site (Figure 1).

Figure 1

Histone modifications mark dynamically regulated genes. In all panels, shades of green indicate active marks, whereas shades of pink represent repressive marks. The orange and yellow colors mark regions of 5 mC and 5 hmC, respectively. (A) The distribution of histone methylation and acetylation marks, along with the degree of methylation, is illustrated across the promoter region, TSS (transcriptional start site), and gene body of a transcriptionally active gene. (B) Histone H3 methylation and DNA methylation are found in the promoter region and surrounding the TSS in transcriptionally repressed genes. (C) Bivalent chromatin domains consist of discrete pockets of histone H3K4me3 within large regions of histone H3K27me3. DNA 5hmC is also found in bivalent domains.

Chromatin modifications mark genomic regions

Many chromatin modifications are associated with either transcriptional activation or repression (Table 1); however, there are certainly exceptions to these assignments. The degree of a modification and where the modifications are located within genic regions affect the transcriptional outcome. Often, multiple enzymes modify a particular amino acid residue within a histone protein. The chromatin-modifying enzymes discussed in this review are listed in Table 1 along with their identified substrates, and Figure 1 serves as a general guide for matching chromatin modifications with transcriptional outcomes. Histone H3K4me2,3, H3R2me2s, H3K9ac, H3K14ac, K3K27ac, and H4K16ac mark euchromatic regions of the genome, particularly transcriptional enhancer and promoter regions10-12 (Figure 1A). Histone H3K36me3 is a mark associated with transcription elongation and is found in the body and 3′ ends of genes (Figure 1A). Methylation of histone H3K79 is largely associated with transcriptionally active genes, with dimethylation and trimethylation found near transcription start sites, and monomethylation occurring widely across active gene bodies.13

View this table:
Table 1

Chromatin-modifying enzymes

Histone H3K9me3, H3K27me1,3, and H4K20me3, together with 5mC are marks of constitutive heterochromatin found in transcriptionally silenced regions of the genome, such as pericentromeric DNA and the inactive X chromosome14,15 (Figure 1B). Histone H3K9me2,3, H3K27me3, and H3R2me2a, as well as 5mC mark facultative heterochromatin and are found in transcriptionally repressed genes that are dynamically regulated16,17 (Figure 1B). The aforementioned repressive chromatin marks do not necessarily coexist at the same promoters. Furthermore, whereas methylated CpG dinucleotides in gene promoters and near transcription start sites often confer a repressive transcriptional state, CpG methylation within gene bodies does not interfere with transcription.18

Genomic regions that contain both transcriptional activating and repressing histone modifications are termed bivalent domains and are prevalent in undifferentiated embryonic stem cells. Bivalent domains consist of small patches of H3K4me3, near the transcriptional start site within larger regions of H3K27me319 (Figure 1C). In addition, 5hmC was recently identified as an additional epigenetic mark enriched at bivalent domains.20

In pluripotent embryonic stem cells, bivalent domains mark genes that are involved in cell fate determination and may help to maintain them in a repressed, yet potentially active, transcriptional state until the onset of differentiation.19 Genome-wide mapping of H3K4me3 and H3K27me3 in hematopoietic stem and progenitor cells revealed the presence of bivalent domains marking genes involved in lineage specification.21,22 Furthermore, the level of H3K4me3 in the progenitor cells correlated significantly with the expression of the marked genes in differentiated cell types.22 These results suggest that the plasticity of the chromatin landscape is crucial for the transition between multipotent and differentiated states.

Writers, erasers, and readers of chromatin modifications

Recent literature is replete with examples demonstrating roles for chromatin-modifying enzymes during hematopoiesis. Moreover, genes encoding these enzymes are frequently disrupted in hematologic malignancies (Table 2).23 Although genome-wide profiling methods provide snapshots of the chromatin landscape during hematopoiesis, genetic mouse models have afforded insights into the roles of chromatin-modifying enzymes during this process.

View this table:
Table 2

Chromatin-modifying enzymes essential for hematopoiesis

DNA methyltransferases (DNMTs) catalyze 5mC

In mammals, the principal enzymes that catalyze cytosine methylation are members of the DNMT1 and DNMT3 families. DNMT1 primarily serves as a maintenance enzyme by copying cytosine methylation patterns from a hemimethylated substrate after DNA replication.24 The DNMT3 family includes 2 active enzymes, DNMT3a and DNMT3b, each of which is further represented by several isoforms. DNMT3a and DNMT3b are referred to as de novo methyltransferases because of their preference to act on unmethylated DNA substrates.25

Conditional deletion of Dnmt1 in hematopoietic stem cells (HSCs) and progenitor cells revealed that Dnmt1 activity is required for early stages of hematopoiesis.26,27 Absence of Dnmt1 activity led to decreased self-renewal capacity26 and a rapid ablation of the HSC pool.27 Furthermore, mouse HSCs lacking Dnmt1 were unable to maintain a sufficient population of myeloid cells because of increased proliferative capacity and decreased differentiation potential of the myeloid progenitor pool.26

Deletion of Dnmt3a and Dnmt3b, either independently or together, in adult mouse HSCs indicated that these enzymes are required for self-renewal but not differentiation or lineage commitment.28 However, a long-term study investigating the role of Dnmt3a in mouse hematopoiesis demonstrated a requirement for Dnmt3a activity in maintaining the differentiation potential of HSCs after serial transplantation into wild-type recipient mice.29 Furthermore, a complex pattern of genomic cytosine methylation was observed in Dnmt3a-deficient HSCs, including hypermethylated regions, that might indicate compensation by other DNMTs.29 Mutations affecting the catalytic activity of DNMT3A have been identified in nearly one third of patients with acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) and are associated with poor clinical outcome, indicating that the function of this enzyme is crucial to maintain normal hematopoiesis.30-32

DNMT1, DNMT3a, and DNMT3b physically and functionally interact with one another, thereby making it more difficult to ascribe the terms maintenance or de novo to any particular enzyme.25 Defining genomic targets of these enzymes will aid in assigning distinct functions. Taken together, these studies indicate that although the mechanisms are not yet clearly defined, appropriate expression and activity of DNA methyltransferases are essential during development and for adult blood homeostasis.

Ten-eleven translocation (TET) enzymes catalyze 5hmC

The TET family of enzymes, composed of TET1, TET2, and TET3, catalyze the oxidation of 5mC to 5hmC, which is an intermediate product of DNA demethylation mechanisms, and is proposed to have functional roles in stem cell biology.4 The TET enzymes have distinct expression patterns, with TET1 expressed in the fetal liver, TET2 in bone marrow, and both TET2 and TET3 in peripheral blood,33 suggesting unique functions for these enzymes during developmental and adult hematopoiesis. Recently, mutations within TET2 were identified as a prevalent occurrence in myeloid malignancies, prompting intense investigations to define the molecular function of TET2 during hematopoiesis.34,35

Mice lacking Tet2 are viable, but hematopoietic malignancies develop within 2 to 6 months.36-39 HSCs obtained from Tet2-deficient mice exhibit enhanced self-renewal capacity and achieve a greater contribution to peripheral blood production in transplanted recipients, compared with wild-type HSCs.36-39 The overall differentiation potential of HSCs was impeded in the absence of Tet2, with a bias toward expansion of monocyte and macrophage lineages.36-38 Disease progression resembling human chronic myelomonocytic leukemia was the most common phenotype observed in the Tet2-null mouse models.36,38,39 In addition, myeloid disorders similar to myeloproliferative disorder–like myeloid leukemia, myeloid leukemia with maturation, and MDS were reported to cause lethality in approximately one third of Tet2-null mice.36

Mutations that disrupt TET2 enzymatic activity and correlate with decreased global 5hmC levels are observed in patients with myeloid malignancies.40 TET2 mutations have also been detected in lymphoid malignancies, suggesting that mutations occur in early hematopoietic progenitors with myeloid and lymphoid potential.39 Moreover, TET2 mutations identified in patients are often monoallelic, indicating that haploinsufficiency contributes to transformation.34,38,39 Heterozygous gain-of-function mutations in the isocitrate dehydrogenase 1 and 2 enzymes, which are prevalent in AML, cause inhibition of TET2 catalytic activity and result in increased global 5mC levels, similar to the effect induced by loss of TET2 function.41

DNA mechanisms of demethylation

The passive loss of cytosine methylation after DNA replication is a widely accepted mechanism for the removal of CpG methylation. The direct removal of a methyl group from DNA is thermodynamically unfavorable.42 However, several pathways recently have been proposed to mediate indirect, yet active, removal of CpG methylation.

TET enzymes catalyze sequential oxidation reactions that convert 5mC to 5hmC, 5fC, and finally to 5caC, which can be removed by DNA repair mechanisms.43 An alternative proposed active mechanism is deamination of 5mC by activation-induced cytidine deaminase, yielding a thymidine base that is subject to subsequent DNA mismatch repair and base excision repair mechanisms.42 Whereas these mechanisms do not directly return the methylated cytosine to an unmodified base, the 5mC functionality is no longer present. Defining DNA demethylation mechanisms is an active area of investigation that is likely to yield exciting results in the future.

DNA methylation readers

Methyl-binding domains (MBDs) recognize and bind to 5mC and are found in a conserved family of proteins that includes MBD1, MBD2, MBD3, MBD4, and MeCP2.44 MBD proteins are generally regarded as mediators of transcriptional repression by recruiting chromatin-modifying enzymes, such as histone deacetylases (HDACs), to regions of highly methylated DNA.44 The MBD proteins are not essential for embryonic development;44 however, transgenic mouse model studies have pointed to a role for Mbd2 in the silencing of embryonic globin gene expression in adult erythroid cells.45,46

The Set and RING finger associated domain within UHRF1, a DNMT1 interacting partner, and the MBD of MBD3 were recently reported as readers of 5hmC.47,48 The UHRF1 Set and RING finger associated domain was previously shown to bind hemimethylated DNA.49 It will be interesting to learn whether these molecular functions overlap or if they occur as distinct targeting mechanisms.

The specificity and affinity of MBD domains for 5mC have made them valuable reagents in defining patterns of global cytosine methylation.50 The MBD domain MBD2 was recently used to affinity-purify methylated DNA fragments from mouse HSCs, CMPs, and erythroblasts to determine changes in 5mC during myeloid differentiation. Using this method, a high level of 5mC was detected in mouse HSCs, with a dramatic loss of global methylation occurring during myeloid differentiation,51 similar to results obtained from a DNA methylation-profiling study performed during erythropoiesis using a bisulfite-based method.52 A similar approach using the MBD domain of MeCP2 was used to detect changes in DNA methylation during later stages of lymphoid differentiation; however, no dramatic change in 5mC patterns were noted,53 suggesting that global changes in DNA methylation accompany early differentiation events during hematopoiesis. Notably, the affinity capture approach can be applied to map the genomic locations of CpG islands using domains that recognize unmethylated CpG sites.54

Histone acetyltransferases (HATs) and deacetylases

A number of HAT enzymes have been identified as regulators of chromatin-templated processes, including gene transcription and DNA repair. Most HATs function within large multimeric complexes that include targeting subunits and additional histone-modifying activities. In mice, homozygous deletion of the HAT Moz leads to reduced numbers of hematopoietic progenitors, although all lineages are represented, indicating that differentiation and lineage commitment are not altered.55,56

Acetylation is a reversible reaction, and HDAC enzymes carry out deacetylation of histones as well as nonhistone substrates. The expression of HDACs 1,2,3 is low in hematopoietic progenitor cells but increases during differentiation. However, overexpression of HDAC1 was shown to inhibit myeloid differentiation.57 HDACs are notoriously linked with hematologic malignancies through their association with leukemogenic fusion proteins, and they mediate aberrant transcriptional repression of genes required for hematopoietic differentiation.58 Small-molecule HDAC inhibitors are used for leukemia therapy independently but are more successful when given in combination with other cytotoxic agents.59 Defining the genomic targets of HDACs will aid in developing inhibitors that specifically target those enzymes modifying genes involved in oncogenesis.

Acetylation readers

Many of the enzymes responsible for “writing” and “erasing” histone modifications contain domains that “read” modifications. Bromodomains are well-characterized modules that bind acetylated lysine residues.60 The bromodomain of the HAT p300/CBP-associated factor binds the acetylated N-terminal tail of H3 to promote transcriptional activation61 (Figure 2). Moreover, an example of a bromodomain indirectly promoting chromatin occupancy of a transcriptional regulator was recently reported. The bromodomain within the bromodomain extra terminal family member, BRD3, interacts with acetylated GATA1 to facilitate its recruitment to genomic targets, including genes involved in erythroid differentiation.62 Bromodomain-mediated interactions are amenable to small-molecule inhibition strategies.63 Several bromodomain extra terminal inhibitors show promise as therapeutic agents for AML through their effective inhibition of leukemia stem and progenitor cell proliferation and blocking of mixed lineage leukemia (MLL)–mediated transformation.64,65

Figure 2

Modification-binding domains interpret histone PTMs. Domains from various histone-interacting proteins are depicted along with the PTMs that they bind on histone H3. Multiple reading domains can interact with a single modified site, as illustrated for histones H3K4, K9, K27, and K36. Interactions may also change depending on the modification present at the site, as shown for acetylation or methylation of histones H3K9 and K36. The colors represent different PTMs and associated binding domains, which are depicted as follows: pink, methylation; green, acetylation.

Lysine methyltransferases (KMTs) and demethylases (KDMs)

The majority of mammalian KMTs characterized to date function within multisubunit complexes and target the N-terminal tail of histone H3, where they coordinate with additional histone-modifying activities to drive either activation or repression of transcription. The MLL methyltransferases carry out methylation of histone H3K4 and are members of the trithorax group family, whereas enzymes that methylate H3K27 belong to the polycomb group (PcG) family. The functional antagonism between these groups of proteins regulates transcription of developmental genes, including the Hox gene cluster, and establishes patterning during embryonic development in vertebrates.

MLL1 is required for viability, as Mll1−/− embryos die by E10.5.66 Yolk sacs derived from Mll1−/− embryos produce fewer and smaller myeloid colonies than yolk sacs derived from Mll1+/+ embryos, indicating that MLL1 is critical for primitive hematopoiesis.67 Mll1 is required for HSC and early progenitor self-renewal during hematopoiesis in the fetal liver68 as well as in the adult bone marrow.69 The human MLL1 gene is often disrupted by chromosomal translocations observed in acute leukemias,70 and defining the molecular mechanisms underlying transformation remains an active area of investigation.71,72 Furthermore, inactivating mutations in other MLL proteins, including MLL2 and MLL3, are observed in various cancers.23

There are 2 well-characterized PcG complexes that incorporate multiple histone-modifying activities to mediate transcriptional repression: polycomb repressive complex 1 and 2 (PRC1 and PRC2). The enhancer of zeste homolog 2 (EZH2) enzyme functions within the PRC2 complex to catalyze dimethylation and trimethylation of histone H3K27, which is then bound by the PRC1 complex to maintain transcriptional repression of PcG target genes. EZH2 silences genes required for cell fate decisions, thus promoting stem cell self-renewal. Fitting with this role, overexpression of Ezh2 enhances the long-term repopulating potential of HSCs after serial transplantations into mice.73 Ezh2 is required for fetal HSC proliferation and erythropoiesis in the developing embryo, but loss of Ezh2 has only a trivial effect on the function of HSCs in the adult bone marrow, primarily their ability to contribute to lymphopoiesis.74

Consistently, Ezh2 activity was previously found to be essential during the early stages of B-cell development but was dispensable for the maturation and activation of pro-B cells.75 Notably, mutations abrogating the catalytic function of EZH2 were recently identified in acute and chronic myeloid malignancies,76-79 whereas gain-of function mutations were identified in B-cell lymphomas80,81,23. Inhibiting the methyltransferase activity of EZH2 in patients with lymphoma carrying activating mutations might be a promising therapeutic endeavor. Treatment with an EZH2-specific small-molecule inhibitor resulted in decreased lymphoid cell proliferation in vitro and in mouse xenograft models.82

A number of point mutations in the polycomb-associated gene addition of sex combs-like 1 (ASXL1) have been identified in patients with MDS or AML and correlate with a poor prognosis.83-86 ASXL1 mutations promote transformation by decreasing the recruitment of PRC2 to leukemogenic target genes, resulting in loss of histone H3K27 methylation and transcriptional repression of those genes.87 Given that gain- or loss-of-function mutations affects various components of the PRC2 complex suggests that altering PRC2 activity and/or H3K27 methylation levels in either direction are detrimental to normal hematopoiesis.

The disruptor of the telomeric silencing 1-like (DOT1L) enzyme methylates the globular domain of histone H3 at K79, which correlates with active transcription.88 Mice deficient for Dot1L exhibit severe anemia and die between E10.5-13.5.89 Erythroid development was specifically inhibited in Dot1L-null mice, and altered H3K79 methylation status and expression of the erythroid regulatory genes, Gata2 and Pu1, were observed.89 Conditional targeting strategies revealed a role for Dot1L in maintaining adult hematopoiesis as well.90,91 Notably, aberrant recruitment of DOT1L by several MLL fusion proteins to genes involved in cellular transformation is a common mechanism underlying MLL-mediated leukemogenesis.13,92 Moreover, DOT1L is a promising therapeutic target in mixed myeloid leukemias in which MLL and/or AF10 oncogenic fusion proteins are expressed.93,94

Histone lysine methylation is dynamic and is regulated by KDM enzymes. Two classes of KDMs acting on histones have been identified: amine oxidases and jumonji C domain–containing enzymes. Lysine-specific demethylase 1 (LSD1) was the first histone KDM identified and exhibits dual substrate specificity for histones H3K4 and H3K9.95,96 LSD1 interacts with transcriptional repressors and demethylates histone H3K4me1,2 yielding an unmodified lysine residue. LSD1 and HDACs1 and 2 interact with the T-cell acute lymphocytic leukemia 1 transcription factor in erythroleukemia cell lines and negatively regulate T-cell acute lymphocytic leukemia 1 target genes to inhibit the onset of erythroid differentiation.97 LSD1 also cooperates with the Gfi-1 transcriptional repressors in a lineage-specific manner to regulate megakaryocytic, granulocytic, and erythroid differentiation.98 Furthermore, depletion of Lsd1 in mice leads to enhanced HSC and progenitor cell proliferation and deficient erythroid differentiation.99 Regulatory roles for LSD1 in defining hematopoietic lineage commitment highlight the importance of restricting histone H3K4 methylation patterns to establish and maintain differentiation programs.

Arginine methyltransferases

Methylation of arginine residues within histone and nonhistone proteins is catalyzed by protein arginine (R) methyltransferases (PRMTs). There are 9 identified enzymes in the mammalian PRMT family (PRMT1-9). They are generally classified as type 1 enzymes (PRMTs 1, 3, 4, 6, and 8), which catalyze asymmetric dimethylation; or type 2 enzymes (PRMTs 5 and 7), which catalyze symmetric dimethylation.100 Methylated arginine residues identified in histones H3 and H4 directly and indirectly regulate transcription.

PRMT1 catalyzes asymmetric dimethylation of histone H4R3 (H4R3me2a), which promotes p300-mediated acetylation of H4K8 and K12, resulting in transcriptional activation.101 PRMT1 interacts with a leukemogenic MLL fusion protein (MLL-EEN) to promote transformation of primary hematopoietic progenitor cells. The oncogenic mechanism is dependent on PRMT1-mediated methylation of histone H4R3 and transcriptional activation of MLL target genes.102 Furthermore, cross-talk occurring between PRMTs and KMTs is necessary to establish appropriate patterns of histone methylation. For instance, PRMT6-mediated methylation of histone H3R2me2a indirectly represses transcription by inhibiting MLL-mediated activity toward histone H3K4,17,103 and by blocking the association of histone H3K4-methyl–binding proteins.104

PRMTs influence transcription via methylation of histone and nonhistone substrates, including transcription factors. In human hematopoietic progenitor cells, PRMT6 physically interacts with runt-related transcription factor 1/AML1 at the promoters of megakaryocytic genes and maintains a chromatin environment that is repressed but primed for transcriptional activation.105 Moreover, PRMT1-mediated methylation of runt-related transcription factor 1/AML1 itself enhances its ability to activate transcription of several target genes during hematopoietic differentiation by inhibiting its association with the SIN3A corepressor complex.106 PRMT1 also interacts with an alternatively spliced version of the AML1-ETO leukemogenic fusion protein, AE9a, and activates transcription of AE9a target genes to enhance proliferation of hematopoietic progenitor cells.107

Lysine and arginine methylation readers

Methyl lysine-binding domains include the plant homeodomain (PHD) finger, chromo, tudor, malignant brain tumor, PWWP, and the ankyrin repeat. Multiple binding domains show an affinity for particular lysine or arginine residues within histone tails; however, binding by different effector molecules depends on whether methylation or acetylation is present (Figure 2). In addition, effector binding also depends on the degree of the modification present on a particular residue, such as me1, me2, me3. These requirements suggest temporal regulation of binding events to ensure that the appropriate interactions occur between the histone substrate and the binding effector molecule.

Histone-modifying enzymes often contain domains that bind modifications. For instance, the histone demethylase JARID1A contains a C-terminal PHD finger that binds its substrate, H3K4me2,3 (Figure 2). Translocations occurring in AML create a JARID1A-NUP98 fusion protein that uses this PHD finger to aberrantly bind PcG-silenced Hox genes and maintain them in a transcriptionally active state, resulting in an arrest of hematopoietic differentiation.108 Furthermore, replacing the JARID1A PHD domain with a PHD domain that reads unmethylated histone H3K4 fails to induce transformation, supporting the idea that induced transcriptional activation of temporally regulated genes is crucial to the leukemogenic mechanism.108 This example demonstrates that aberrant genomic targeting of a histone modification–reading domain is sufficient to drive transformation.

The conserved PHD domains within MLL1 are lost after leukemogenic translocations.109 It was recently shown that MLL1 PHD3 binds histone H3K4me2/3 and is required for localization of wild-type MLL1 to target gene promoters to activate transcription.110,111 Introduction of the third PHD finger (PHD3) into the MLL-ENL fusion protein blocks aberrant HoxC8 gene activation in an HDAC-dependent manner and subsequently inhibits hematopoietic progenitor transformation.112 Similarly, adding multiple PHD fingers to the MLL-AF9 fusion protein inhibits association with the HoxA9 locus and immortalization of hematopoietic progenitors.109 Taken together, it is evident that loss of the PHD fingers is a common contributing factor to MLL1-mediated leukemogenesis.

Epigenetic mechanisms in normal hematology and in hematologic malignancies

Genomic loci encoding chromatin-modifying enzymes are frequently disrupted in human cancers, and these alterations are especially prevalent in hematologic disorders. It is noteworthy that many modification readers promote transformation by cooperating with oncogenic enzymes. Loss or gain of conserved catalytic domains and/or modification-reading domains results in deregulated enzyme activity toward histone and nonhistone substrates that ultimately affects chromatin structure and function to promote leukemogenesis. In addition, fusion proteins created by translocations often lead to altered protein-protein interactions that target histone-modifying activities to inappropriate genomic targets. One significant point that remains is how these enzymes are targeted to specific genomic loci. Patterns of DNA methylation and histone modification are frequently interdependent because of functional interactions between the enzymes that modify DNA and histones. It is critical to define common DNA methylation and histone modification patterns, in addition to downstream gene targets affected by genetic alterations in the writers, erasers, and readers of chromatin modifications as we look toward the future design of molecular-based therapies to treat hematologic malignancies.

Authorship

Contribution: J.S.B. and S.Y.R.D. wrote the manuscript.

Conflict-of-interest disclosure: The authors declare no competing financial interests.

Correspondence: Sharon Dent, Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center Science Park, PO Box 389, Smithville, TX 78957; e-mail sroth{at}mdanderson.org.

Acknowledgments

The authors thank Elizabeth McIvor for helpful comments and discussion on the manuscript. The authors also thank Leisa McCord for assistance with the artwork.

This work was supported by the Cancer Prevention Research Institute of Texas (CPRIT) RP100429 to S.Y.R.D.

  • Submitted October 5, 2012.
  • Accepted December 18, 2012.

References

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