Blood Journal
Leading the way in experimental and clinical research in hematology

Savino B, Castor MG, Caronni N, et al. Control of murine Ly6Chigh monocyte traffic and immunosuppressive activities by atypical chemokine receptor D6. Blood. 2012;119(22):5250-5260.

On page 5252 in the 31 May 2012 issue, there is an error in flow cytometry dot plots that represent F4/80+ and PDCA-1+ cells in Figure 1C. An error during the assembly of the original Figure 1C caused the exclusion of the F4/80 dot plot and a duplicate inclusion of the PDCA-1 dot plot. The gating strategy is now reported in a new Figure 1C, which has been assembled using dot plots from an independent analysis. The corrected Figure 1 is shown.

Figure 1

Lack of D6 on the nonhematopoietic compartments causes increased number of inflammatory monocytes. (A) Percentage in CD45+ gate (left panel) and fold increase of absolute number (right panel) of CD11b+/Ly6Chigh monocytes recovered from blood in D6/ (black bar) versus WT mice (white bar). (B) Fold increase of percentage of CD11b+/Ly6Chigh monocytes in total BM CD45+ leukocytes of D6/ (black bar) and WT mice (white bar). (C) Gating strategy for evaluating the percentage and absolute numbers of leukocytes in mouse spleen. Dead cells, doublets, and CD3+, CD19+, and NK1.1+ cells were excluded for further analysis. Cell labeling with anti-CD11b and CD11c allows identification of myeloid DCs (R1: CD11b+/CD11chigh), red pulp macrophages (R2: F4/80+), and plasmacytoid DCs (R3: PDCA-1+) in the CD11cdim/CD11bdim gate. Inflammatory monocytes (R4) and neutrophils (R5) are distinguished within the CD11bhigh population as SSClow/CD115+/Ly6Chigh and SSChigh/CD115/Ly6Ghigh, respectively. Gates are based on isotype controls. (D) Fold increase of the absolute number of cells in spleen of D6/ (black bar) versus WT (white bar) mice based on gated strategy used in panel C. (E) Fold increase of the absolute number of CD11b+/Ly6Chigh monocytes in blood of D6/ (Ly5.2) mice reconstituted with WT Ly5.1 or D6/ BM cells and in WT mice (Ly5.1) reconstituted with D6/ BM cells versus WT mice (Ly5.1) reconstituted with WT mice (Ly5.2). In all panels, data are representative of 4 independent experiments (n = 10 mice/experiment). *P < .05. **P < .005.