Blood Journal
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Brief report

Distinct platelet packaging, release, and surface expression of proangiogenic and antiangiogenic factors on different platelet stimuli

  1. Madhumita Chatterjee1,
  2. Zhangsen Huang1,2,
  3. Wei Zhang1,
  4. Lei Jiang2,
  5. Kjell Hultenby3,
  6. Linjing Zhu1,
  7. Hu Hu2,
  8. Gunnar P. Nilsson4, and
  9. Nailin Li1
  1. 1Department of Medicine, Clinical Pharmacology Unit, Karolinska Institutet at Karolinska University Hospital (Solna), Stockholm, Sweden;
  2. 2Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China;
  3. 3Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital (Huddinge), Stockholm, Sweden; and
  4. 4Clinical Immunology and Allergy Unit, Karolinska Institutet at Karolinska University Hospital (Solna), Stockholm, Sweden

Abstract

The present study characterized platelet secretion and surface expression of proangiogenic stromal cell-derived factor-1α (SDF-1α) and vascular endothelial growth factor (VEGF) and antiangiogenic PF4 and endostatin on activation. The angiogenic factors presented in randomly distributed granules in resting platelets, which were peripherized on activation. Confocal and immunogold electron microscopy demonstrated that SDF-1α/CXCL12 and PF4/CXCL4 mostly present in different granules. Platelet activation induced marked SDF-1α and endostatin but mild PF4 or no VEGF surface expression. PAR1-activating peptide (PAR1-AP), adenosine diphosphate (via P2Y1/P2Y12), and glycoprotein VI-targeting collagen-related peptide induced massive SDF-1α and VEGF but modest PF4 or no endostatin release. In contrast, PAR4-AP triggered marked PF4 and sole endostatin release but limited SDF-1α or VEGF secretion. Distinct platelet release of SDF-1α and endostatin involved different engagements of intracellular signaling pathways. In conclusion, different platelet stimuli evoke distinct secretion and surface expression of proangiogenic and antiangiogenic factors. PAR1, adenosine diphosphate, and glycoprotein VI stimulation favors proangiogenic, whereas PAR4 promotes antiangiogenic, factor release.

  • Submitted December 21, 2010.
  • Accepted January 30, 2011.
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