Blood Journal
Leading the way in experimental and clinical research in hematology

MyD88-dependent TLR4 signaling is selectively impaired in alveolar macrophages from asymptomatic HIV+ persons

  1. Souvenir D. Tachado1,
  2. Xin Li1,
  3. Medhavi Bole1,
  4. Katharine Swan1,
  5. Asha Anandaiah1,
  6. Naimish R. Patel1, and
  7. Henry Koziel1
  1. 1Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA

Abstract

Alveolar macrophages (AMs) are the predominant effector cell in the lungs and contribute to a critical first line of defense against bacterial pathogens through recognition by pattern recognition receptors such as Toll-like receptor 4 (TLR4). TLR4-mediated tumor necrosis factor α (TNFα) release is significantly impaired in HIV+ macrophages, but whether HIV impairs myeloid differentiation factor 88 (MyD88)–dependent and/or MyD-independent TLR4 signaling pathways in human macrophages is not known. Comparing human U937 macrophages with HIV+ U1 macrophages (HIV-infected U937 subclone), the current study shows that HIV infection is associated with impaired macrophage TLR4-mediated signaling, specifically targeting the MyD88-dependent TLR4-mediated signaling pathway (reduced MyD88–interleukin-1 receptor–associated kinase [IRAK] interaction, IRAK phosphorylation, nuclear factor [NF]–κB nuclear translocation, and TNFα release) while preserving the MyD88-independent TLR4-mediated signaling pathway (preserved STAT1 phosphorylation, interferon regulatory factor [IRF] nuclear translocation, and interleukin-10 [IL-10] and RANTES release). Extracellular TLR4 signaling complex was intact (similar levels of CD14 and MD2), and similar patterns of response were observed in clinically relevant AMs from healthy and asymptomatic HIV+ persons at high clinical risk of pneumonia. Taken together, these data support the concept that chronic HIV infection is associated with specific and targeted disruption of critical macrophage TLR4 signaling, which in turn may contribute to disease pathogenesis of bacterial pneumonia.

  • Submitted October 23, 2009.
  • Accepted February 12, 2010.
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