Advertisement

Defective killing of dendritic cells by autologous natural killer cells from acute myeloid leukemia patients

Cyril Fauriat, Alessandro Moretta, Daniel Olive and Régis T. Costello

Article Figures & Data

Figures

  • Figure 1.

    NCRdull AML-NK cell killing of normal monocyte-derived DCs. The expression of NKp30 was analyzed by flow cytometry on NK cells obtained from 3 healthy donors (NCRbright[A]) or 7 AML patients (1 of 2 patients with NCRbright phenotype [B], or 1 of 5 patients with NCRdull phenotype [C]) as described in “Study design.” Black histograms represent the isotype-matched negative controls; and white histograms, the PE-conjugated anti-NKp30 mAb staining. On the corresponding diagrams, the 3 different NK cells (ie, normal NCRbright NK, NCRbright AML-NK, and NCRdull AML-NK) were assessed for cytolytic activity against the P815 murine cell line (column i), immature Mo-DCs (iDC, column ii), and mature Mo-DCs (mDC, column iii). ▪ represent the redirected killing with the IgG1 anti-CD16 mAb; •, the redirected killing with the IgG1 anti-NKp30 mAb; and ♦, the redirected killing with an irrelevant IgG1 mAb. ▵ represent NK cells against iDCs, and the crosses represent the natural killing of NK cells against iDCs blocked with an IgM anti-NKp30 mAb. Grey circles represent the natural killing of NK cells against mDCs, and ○ represent the blocking of HLA class I engagement by IgM anti-HLA class I mAb. These data are from 1 experiment of 5 representative and independent performed. Error bars indicate ± standard deviation (SD).

  • Figure 2.

    NCRdull AML-NK cell killing of leukemia-derived DCs. NCRdull AML-NK cells were obtained and amplified as described in “Study design” and assessed for spontaneous cytolytic activity against autologous (A) or allogeneic (B) iDCs (▴) and mDCs (•). These data are from 1 experiment of 3 representative and independent performed. Error bars indicate ± standard deviation (SD). E/T indicates effector-target ratio.