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Recently, Croop et al1 reported the feasibility of mobilizing and collecting peripheral blood CD34+ progenitor cells from patients with Fanconi anemia. The authors performed successful apheresis collection in 6 of 8 patients. They showed that prolonged granulocyte colony-stimulating factor (G-CSF) administration (median 10 ± 4 d) and days of apheresis (4 ± 3 d; range, 2-8 d) were required and that such procedure led to significant adverse events. This study supported evidence that patients with Fanconi anemia can be mobilized, but the authors did not answer the question of whether these hematopoietic progenitors were functional and could efficiently participate to autologous reconstitution after infusion. In this scope, data concerning granulocyte-macrophage colony-forming unit (GM-CFU) assays would have been of great interest.

We would like to report our experience with G-CSF–mobilized peripheral blood CD34+ cells (n = 4) or bone marrow (BM) harvest (n = 2) in patients with Fanconi anemia, using a standard collection method. At the time of harvest, none of the patients had criteria of severe aplastic anemia (defined by hemoglobin level below 80 g/L [8 g/dL], absolute neutrophil count below 0.5 × 109/L, and platelet count below 20 × 109/L) and were transfusion independent (Table1). All 4 mobilized patients, with a mean age of 17.3 y (range, 4-31 y), received G-CSF at the daily dose of 10 μg/kg for 5 days. Two of them failed to mobilize, while the other 2 underwent apheresis with a number of initial peripheral blood CD34+counts of 9.4/μL and 9.8/μL. Within 2 days of apheresis, the numbers of total nucleated cells collected in these 2 patients were 84.86 × 109/kg and 90.14 × 109/kg and the numbers of CD34+ cells were 1.53 × 106/kg and 0.9 × 106/kg. GM-CFU assays were performed by seeding 1 × 103 CD34+ cells in a conventional methylcellulose culture assay in order to determine myeloid progenitors cell growth. Interestingly, CD34+ progenitor cells failed to generate GM-CFU colonies with numbers of 0.42 × 104 or 1 × 104 GM-CFU/kg patient weight, thus suggesting a low clonogenic capacity of CD34+ cells even in patients without severe bone marrow failure. We determined that CD34+ cells from Fanconi anemia patients have 5 to 12 times lower levels of GM-CFU/kg, as compared with 72 patients with hematologic malignancies for whom apheresis, performed with the same standard conditions, led to a median of 1.1 × 106CD34+/kg (median GM-CFU/kg, 5.1 × 104). The same pattern of dramatic decrease in CD34+ cells (0.03 × 106/kg and 0.12 × 106/kg) and GM-CFU (0 × 104/kg) was observed in both patients that underwent BM harvest. These results contrast with a relatively normal blood count that cannot be predictive of CD34 cell recovery or functionality.

View this table:
Table 1.

Patients characteristics and analysis of collection and clonogenicity of bone marrow or peripheral blood progenitor cells

Similar to a murine model of FANCC disruption clearly demonstrating a reduced repopulating ability of hematopoietic stem cells,2our results tend to support the hypothesis of a qualitative and quantitative defect of hematopoietic progenitor cell compartment.

References