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Article Figures & Data

Tables

  • Table 1.

    Results from patients with thrombocytopenia

    CasePlatelet count (× 109/L)Direct PIFT T/C ratio*OD values
    Direct MAIPAIndirect MAIPA
    ControlPatientControlPatient
    123.60.131.850.120.151-153
    266.80.090.230.010.10
    3722.8ntnt0.010.21
    412ntntnt0.010.21
    5358.80.011.630.020.08
    6583.90.131.070.120.29
    7601.90.03> 3.50.020.26
    8napositiventnt0.230.60
    9napositiventnt0.020.18
    10nanantnt0.090.151-153
    0.091-159 0.541-159
    • * Flow cytometry test results as a ratio of the test result (T) compared with the control (C). Samples 8 and 9 were tested by microscopy.

    • Results from control healthy donor platelets and patient's own platelets tested in parallel, using SW16 anti-GPV as capture mAb.

    • Average result from testing at least 3 cells previously incubated with the blood group AB control sera and patient's serum tested in parallel, using SW16 anti-GPV as capture mAb.

    • F1-153 Result not above cut-off of twice the control result.

    • F1-159 Autoantibodies were eluted from the patient's platelets and tested by MAIPA.

    • na, not available, archive samples referred to in the text; nt, not tested.

  • Table 2.

    Indirect MAIPA with serum and IgG fractions, in the absence and presence of gold

    CaseSerumPurified IgG
    Native sampleGold (μg/mL)*Native sampleGold (μg/mL)
    0.11100.1110
    Control 0.01 0.000.000.000.150.120.130.12
    20.430.340.360.300.490.410.230.39
    31.391.141.931.040.460.310.350.23
    40.380.280.390.230.340.190.270.17
    70.330.240.240.180.560.480.480.53
    81.120.951.020.971.741.401.451.32
    • Native samples are samples without gold added.

    • * Final concentration of gold during all stages of MAIPA. For clarity, results using 100 μg/mL are not shown.

    • Negative control AB serum or IgG fraction from AB serum.

    • ELISA OD values. Results are from a representative experiment, derived from duplicate testing against platelets from 2 donors.