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 Previous Article | Table of Contents | Next Article 
Production of Interleukin-10 by Granulocyte Colony-Stimulating
Factor-Mobilized Blood Products: A Mechanism for Monocyte-Mediated
Suppression of T-Cell Proliferation
Marco Mielcarek,
Lynn Graf,
Gretchen Johnson, and
Beverly Torok-Storb
From the Transplantation Biology Program, Clinical Research Division,
Fred Hutchinson Cancer Research Center, Seattle, WA.
Previous reports showed that granulocyte colony-stimulating factor
(G-CSF)-mobilized peripheral blood mononuclear cells (G-PBMC) are
hyporesponsive to alloantigen compared with control PBMC. In the
current study, neutralizing antibodies to interleukin-10 (IL-10)
increased the proliferative response of G-PBMC to alloantigen by
50.14% (± 12.79%; n = 8), whereas the proliferative response of
control PBMC was not affected. The inhibition of OKT3-stimulated CD4
cell proliferation by G-PBMC-derived CD14+ cells could
also be abrogated by the addition of IL-10 neutralizing antibodies.
Further, IL-10 levels correlated with the number of CD14 cells in these
cultures. Constitutive IL-10 mRNA levels detected by quantitative
reverse transcriptase-polymerase chain reaction (RT-PCR) were 10-fold
higher in G-PBMC compared with control PBMC. This translated into
significantly higher IL-10 levels after 24-hour lipopolysaccharide
(LPS) stimulation of G-PBMC compared with control PBMC (P = .036). IL-10 mRNA levels were also fivefold higher in isolated
G-PBMC-derived CD14 cells compared with control CD14 cells. This
corresponded to increased constitutive production of IL-10 by isolated
G-PBMC-derived CD14 cells compared with control CD14 cells (357.2 ± 104.5 v 51.7 ± 30.5, P = .051). In conclusion, these data suggest that monocytes contained within G-PBMC, which, in
comparison to marrow, are increased in absolute number and relative
proportion to T cells, may suppress T-cell responsiveness by secretion
of IL-10.
Blood, Vol. 92 No. 1 (July 1), 1998:
pp. 215-222
© 1998 by The American Society of Hematology.
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