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Analysis of VH gene expression in CD5+ and CD5- B-cell chronic lymphocytic
leukemia
K Maloum, F Davi, C Magnac, O Pritsch, E McIntyre, F Valensi, JL Binet, H Merle- Beral and G Dighiero
Department of d'Hematologie, CHU Pitie-Salpetriere, Paris.
In contrast to highly mutated follicular lymphomas and multiple myelomas,
chronic lymphocytic leukemias (CLLs) frequently express VH genes in
germline configuration. It is currently unclear whether this difference is
related to the expression of CD5 or to the differentiation stage of the B
cell when malignant transformation occurs. We have studied the VH sequence
of 11 cases of CD5- B-CLL to address the question whether CD5- B-CLL are
derived from naive pregerminal B cells (low mutation pattern) or from
germinal center- derived memory B cells (high mutation pattern). Among the
12 detected rearrangements (2 distinct rearrangements in 1 case) VH1 family
was found in 2, VH2 in 2, VH3 in 4, and VH4 in 4. Nine different VH genes
were detected among the 12 rearrangements, including 2 cases expressing
V1-69 (51p1) and 1 case expressing V4-39 (VH4.18), previously reported to
be overexpressed in CD5+ B-CLL. A higher mutation pattern, following a
random distribution, was observed when compared with classical CD5+ B- CLL.
However, as reported in normal B cells, these results appeared to be
related to membrane Ig phenotype (less mutations in membrane mu
delta-expressing forms in leukemias expressing exclusively membrane mu).
Overall, the differences found when comparing the mutational profile with
classical CD5+ B-CLL were not clearcut and might be explained more by the
membrane isotype (mu v mu delta) than by CD5 expression.
Volume 86,
Issue 10,
pp. 3883-3890,
11/15/1995
Copyright © 1995 by The American Society of Hematology
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