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Establishment and erythroid differentiation of a cytokine-dependent human
leukemic cell line F-36: a parental line requiring granulocyte- macrophage
colony-stimulating factor or interleukin-3, and a subline requiring
erythropoietin
S Chiba, F Takaku, T Tange, K Shibuya, C Misawa, K Sasaki, K Miyagawa, Y Yazaki and H Hirai
Third Department of Internal Medicine, University of Tokyo, Japan.
We have established a new nonlymphoid leukemic cell ine from a patient with
myelodysplastic syndrome (MDS), which progressed to overt leukemia. The
parental cell line and a subline derived from this line have absolute
dependency on several cytokines for their long-term survival and growth.
The parental line designated F-36P requires granulocyte-macrophage
colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3) for continuous
growth, while a subline designated F-36E can be maintained in the presence
of erythropoietin (Epo) alone. When these cytokines are depleted, both the
parental and the subline cells die within several days, even in medium
supplemented with fetal calf serum (FCS). F-36E, maintained in the presence
of Epo, constitutively synthesizes hemoglobin at a significant level.
F-36P, which is usually maintained in the presence of GM-CSF or IL-3, can
be induced to synthesize hemoglobin when GM-CSF or IL-3 is substituted by
Epo. The surface marker profile shows that the F-36P cells are positive for
the leukocyte common antigen (CD45) and some common multilineage markers
such as CD13, CD33, and CD34, and negative for T- and B-cell antigens and
mature myelomonocytic antigens. However, some monoclonal antibodies
recognizing erythroid and platelet glycoproteins react with these cells.
Thus, this cell line has a multilineage phenotype, suggesting that the
transformation event occurred in a multipotent stem cell. It is also
evident that the F-36 cells can be induced to differentiate into the
erythroid lineage in the presence of Epo. This, to our knowledge, is the
first description of a human leukemic cell line that can be stimulated to
synthesize hemoglobin by Epo.
Volume 78,
Issue 9,
pp. 2261-2268,
11/01/1991
Copyright © 1991 by The American Society of Hematology
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