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A glycoprotein inhibitor of in vitro granulopoiesis associated with AIDS
IZ Leiderman, ML Greenberg, BR Adelsberg and FP Siegal
Division of Clinical Immunology, Mount Sinai School of Medicine (CUNY).
Patients infected with the human immunodeficiency virus (HIV) often present
with neutropenia. To elucidate the mechanism(s) of this HIV- related
neutropenia, we assessed the proliferative capacity of the
granulocyte-macrophage progenitor cell (CFU-GM) from the bone marrow (BM)
of 78 patients within the AIDS spectrum manifesting symptoms or signs
related to HIV infection. Of these, 70 had a significant deficit in the
growth of this committed progenitor when compared with normal controls (P
less than .01). Further analysis revealed that the nucleated bone marrow
cells from AIDS and AIDS-related complex (ARC) patients inhibited the
growth of CFU-GMs from normal individuals when cocultured in agar (P less
than .001). Control CFU-GMs were also inhibited when they were cultured
over feeder layers containing patients' BM cells (P less than .001).
Conditioned media obtained from the liquid culture of patients' BM cells
did not inhibit normal control CFU-GM growth to a degree different from
that of the cells themselves (P greater than .4). Analysis of these
conditioned media by polyacrylamide gel electrophoresis (PAGE) revealed a
unique glycoprotein (gp) with a mol wt of 84 kd. Further studies revealed
that this gp possessed the inhibitory activity. These data suggest that
this gp may be an important factor in HIV-related neutropenia. The presence
of gp84 was independent of drugs administered to the patients.
Volume 70,
Issue 5,
pp. 1267-1272,
11/01/1987
Copyright © 1987 by The American Society of Hematology
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