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Microrheologic investigation of erythrocyte deformability in diabetes
mellitus
JR Williamson, RA Gardner, CW Boylan, GL Carroll, K Chang, JS Marvel, B Gonen, C Kilo, R Tran-Son-Tay and SP Sutera
This study was undertaken to determine whether diabetes alters the
viscoelastic properties of erythrocytes. The oldest and youngest 10%
fractions of circulating red cells were separated by centrifugation of
freshly drawn blood obtained from ten diabetics with disease of one to 20
years' duration and from an equal number of age- and sex-matched control
subjects. Cells from each fraction were suspended in phosphate- buffered
saline, and their rheologic behavior was examined in a rheoscope. The
elongation of cells, the percentage of cells that tank- treaded in response
to shear stress, tank-treading frequency, and the rate of recovery of cell
shape upon cessation of shear stress were determined in the oldest and
youngest 10% of cells for diabetics as well as for controls. All four
parameters were virtually identical for diabetics and controls. Additional
aliquots of cells were taken for assessment of nonenzymatic glucosylation
of hemoglobin and cell membrane protein. The absence of any measurable
difference in rheologic behavior of cells from diabetic and control
subjects, despite substantial differences in nonenzymatic glucosylation of
hemoglobin and cell membrane proteins, suggests that the magnitude of
glucosylation observed in these cellular constituents does not alter the
viscoelastic properties of the cells. The implication of these observations
is that microvascular complications of diabetes are not attributable to
altered deformability of red cells.
Volume 65,
Issue 2,
pp. 283-288,
02/01/1985
Copyright © 1985 by The American Society of Hematology
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