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Blood, 15 June 2006, Vol. 107, No. 12, pp. 4817-4824. Prepublished online as a Blood First Edition Paper on February 21, 2006; DOI 10.1182/blood-2006-01-0057. This Article Full Text (PDF) All Versions of this Article: 2006-01-0057v1 107/12/4817    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chan, J. L Articles by Rameshwar, P. Search for Related Content PubMed PubMed Citation Articles by Chan, J. L Articles by Rameshwar, P. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 6, 2006 Accepted February 6, 2006 Antigen presenting property of mesenchymal stem cells occurs during a narrow window at low levels of interferon- Jennifer L Chan, Katherine C Tang, Anoop P Patel, Larissa M Bonilla, Nicola Pierobon, Nicholas M Ponzio, and Pranela Rameshwar* Graduate School of Biomedical Sciences, UMDNJ, Newark, NJ, USA; Department of Pathology and Laboratory Medicine, NJMS-UMDNJ, Newark, NJ, USA Graduate School of Biomedical Sciences, UMDNJ, Newark, NJ, USA Department or Pharmacology and Physiology, NJMS-UMDNJ, Newark, NJ, USA Department of Pathology and Laboratory Medicine, NJMS-UMDNJ, Newark, NJ, USA Department of Medicine-Hematology/Oncology, NJMS-UMDNJ, Newark, NY, USA * Corresponding author; email: rameshwa{at}umdnj.edu. Mesenchymal stem cells (MSCs) are mostly found around the vasculature system of the adult bone marrow (BM). They function as immune suppressors, express MHC-II, are phagocytic, and support T-cell cytotoxicity. We hypothesize that these contradictory properties of MSCs are important for BM homeostasis and occur partly through antigen presentation (APC) within a narrow window. Indeed, we have verified APC functions of MSCs to recall antigens, C. albicans and T. toxoid. The target cells have been identified to be CD4+ T-cells. APC assays with IFN-knockdown MSCs and with anti-IFN receptor confirmed that MHC-II expression requires autocrine stimulation by IFN. During APC functions, as IFNlevels become elevated, there was a concomitant decrease in MHC-II on MSCs. This observation was correlated with flow cytometry studies showing a gradual decrease in MHC-II expression as IFN levels were increased. The reduced levels of MHC-II correlated with losses in their allogeneic potential, as indicated in mixed lymphocyte reaction. In summary, endogenous and low levels of IFN are required for MHC-II expression on MSCs, and for APC functions. APC functions occur during a narrow window before IFN levels are increased. The study has implications for BM protection against infection and exacerbated inflammatory responses CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. Parekkadan, A. W. Tilles, and M. L. Yarmush Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Autoimmune Enteropathy Independently of Regulatory T Cells Stem Cells, July 1, 2008; 26(7): 1913 - 1919. [Abstract] [Full Text] [PDF] F. Morandi, L. Raffaghello, G. Bianchi, F. Meloni, A. Salis, E. Millo, S. Ferrone, V. Barnaba, and V. Pistoia Immunogenicity of Human Mesenchymal Stem Cells in HLA-Class I-Restricted T-Cell Responses Against Viral or Tumor-Associated Antigens Stem Cells, May 1, 2008; 26(5): 1275 - 1287. [Abstract] [Full Text] [PDF] K. C. Tang, K. A. Trzaska, S. V. Smirnov, S. V. Kotenko, S. K. Schwander, J. J. Ellner, and P. Rameshwar Down-Regulation of MHC II in Mesenchymal Stem Cells at High IFN-{gamma} Can Be Partly Explained by Cytoplasmic Retention of CIITA J. Immunol., February 1, 2008; 180(3): 1826 - 1833. [Abstract] [Full Text] [PDF] E. Traggiai, S. Volpi, F. Schena, M. Gattorno, F. Ferlito, L. Moretta, and A. Martini Bone Marrow-Derived Mesenchymal Stem Cells Induce Both Polyclonal Expansion and Differentiation of B Cells Isolated from Healthy Donors and Systemic Lupus Erythematosus Patients Stem Cells, February 1, 2008; 26(2): 562 - 569. [Abstract] [Full Text] [PDF] A. J. Nauta and W. E. Fibbe Immunomodulatory properties of mesenchymal stromal cells Blood, November 15, 2007; 110(10): 3499 - 3506. [Abstract] [Full Text] [PDF] S. J. Greco and P. Rameshwar Enhancing Effect of IL-1{alpha} on Neurogenesis from Adult Human Mesenchymal Stem Cells: Implication for Inflammatory Mediators in Regenerative Medicine J. 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