SEARCH:   Advanced

Blood, 1 November 2005, Vol. 106, No. 9, pp. 3074-3081. Prepublished online as a Blood First Edition Paper on June 7, 2005; DOI 10.1182/blood-2004-10-4094. This Article Full Text (PDF) All Versions of this Article: 2004-10-4094v1 106/9/3074    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zilber, M.-T. Articles by Gelin, C. Search for Related Content PubMed PubMed Citation Articles by Zilber, M.-T. Articles by Gelin, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 26, 2004 Accepted May 27, 2005 MHC class II/CD38/CD9: a lipid raft-dependent signaling complex in human monocytes Marie-Therese Zilber, Niclas Setterblad, Thierry Vasselon, Christelle Doliger, Nuala Mooney, Dominique Charron, and Catherine Gelin* Inserm U662, Institut d'Hematologie, Hopital Saint-Louis, Paris, France Service Commun d'Imagerie, Institut d'Hematologie, Hopital Saint-Louis, Paris, France * Corresponding author; email: gelin{at}histo.chu-stlouis.fr. Despite a lack of signaling motifs in their cytoplasmic domain, MHC class II molecules trigger a variety of intracellular signals that regulate antigen-presenting cells function. They thus may use associated effector molecules as demonstrated on B cells and dendritic cells. The starting point of this study comes from our previous work, which demonstrated that the ecto-enzyme CD38 is functionally linked to MHC class II molecules. We report that CD38 and HLA-DR are functionally and physically associated in lipid rafts microdomains of cell surface monocytes and that the integrity of these domains is necessary for the HLA-DR and CD38 signaling events. Moreover we identified the tetraspanin CD9 molecule as a partner of the CD38/HLA-DR complex, and demonstrated that HLA-DR, CD38 and CD9 share a common pathway of tyrosine kinase activation in human monocytes. The analysis of conjugates formation between monocytes presenting superantigen and T cells shows the active participation of CD9 and HLA-DR on the monocyte surface. Together, these observations demonstrate the presence of a CD38 and HLA-DR signaling complex within tetraspanin-containing lipid rafts, and the functional impact of their molecular partner CD9 in antigen-presentation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Malavasi, S. Deaglio, A. Funaro, E. Ferrero, A. L. Horenstein, E. Ortolan, T. Vaisitti, and S. Aydin Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology Physiol Rev, July 1, 2008; 88(3): 841 - 886. [Abstract] [Full Text] [PDF] A. A. Khan, S. Martin, and B. Saha SEB-induced signaling in macrophages leads to biphasic TNF-{alpha} J. Leukoc. Biol., June 1, 2008; 83(6): 1363 - 1369. [Abstract] [Full Text] [PDF] P. Munoz, M. Mittelbrunn, H. de la Fuente, M. Perez-Martinez, A. Garcia-Perez, A. Ariza-Veguillas, F. Malavasi, M. Zubiaur, F. Sanchez-Madrid, and J. Sancho Antigen-induced clustering of surface CD38 and recruitment of intracellular CD38 to the immunologic synapse Blood, April 1, 2008; 111(7): 3653 - 3664. [Abstract] [Full Text] [PDF] I. Sloma, M.-T. Zilber, T. Vasselon, N. Setterblad, M. Cavallari, L. Mori, G. De Libero, D. Charron, N. Mooney, and C. Gelin Regulation of CD1a Surface Expression and Antigen Presentation by Invariant Chain and Lipid Rafts J. Immunol., January 15, 2008; 180(2): 980 - 987. [Abstract] [Full Text] [PDF] S. Deaglio, T. Vaisitti, R. Billington, L. Bergui, P. Omede', A. A. Genazzani, and F. Malavasi CD38/CD19: a lipid raft-dependent signaling complex in human B cells Blood, June 15, 2007; 109(12): 5390 - 5398. [Abstract] [Full Text] [PDF] J. J. Unternaehrer, A. Chow, M. Pypaert, K. Inaba, and I. Mellman The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface PNAS, January 2, 2007; 104(1): 234 - 239. [Abstract] [Full Text] [PDF] E. Ortolan, E. V. Tibaldi, B. Ferranti, L. Lavagno, G. Garbarino, R. Notaro, L. Luzzatto, F. Malavasi, and A. Funaro CD157 plays a pivotal role in neutrophil transendothelial migration Blood, December 15, 2006; 108(13): 4214 - 4222. [Abstract] [Full Text] [PDF] N. J. Poloso, L. K. Denzin, and P. A. Roche CDw78 Defines MHC Class II-Peptide Complexes That Require Ii Chain-Dependent Lysosomal Trafficking, Not Localization to a Specific Tetraspanin Membrane Microdomain J. Immunol., October 15, 2006; 177(8): 5451 - 5458. [Abstract] [Full Text] [PDF] S. Deaglio, T. Vaisitti, S. Aydin, E. Ferrero, and F. Malavasi In-tandem insight from basic science combined with clinical research: CD38 as both marker and key component of the pathogenetic network underlying chronic lymphocytic leukemia Blood, August 15, 2006; 108(4): 1135 - 1144. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020